Fluorescence Polarization Assays in Signal Transduction Discovery

Title: Fluorescence Polarization Assays in Signal Transduction Discovery

Volume: 6 Issue: 3

Author(s): J. Richard Sportsman, Janet Daijo and Elizabeth A. Gaudet

Affiliation:

Keywords: Fluorescence Polarization, adenosine monophosphate, phosphodiesterases

Abstract: Fluorescence polarization (FP) has become widely employed for high throughput screening used in pharmaceutical drug discovery. Assays of important signal transduction targets are now adapted to FP. In this review we examine assays for cyclic adenosine monophosphate, phosphodiesterases, and protein kinases and phosphatases using FP competitive immunoassays and a direct enzymatic method called IMAP.

Cite this article as:

Sportsman Richard J., Daijo Janet and Gaudet A. Elizabeth, Fluorescence Polarization Assays in Signal Transduction Discovery, Combinatorial Chemistry & High Throughput Screening 2003; 6 (3) . https://dx.doi.org/10.2174/138620703106298374

DOI https://dx.doi.org/10.2174/138620703106298374	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402