Fluorescence Polarization Assays in Signal Transduction Discovery

J.   Richard   Sportsman; Janet      Daijo; Elizabeth   A.   Gaudet

doi:10.2174/138620703106298374

Abstract

Fluorescence polarization (FP) has become widely employed for high throughput screening used in pharmaceutical drug discovery. Assays of important signal transduction targets are now adapted to FP. In this review we examine assays for cyclic adenosine monophosphate, phosphodiesterases, and protein kinases and phosphatases using FP competitive immunoassays and a direct enzymatic method called IMAP.

Keywords: Fluorescence Polarization, adenosine monophosphate, phosphodiesterases

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Combinatorial Chemistry & High Throughput Screening

Title: Fluorescence Polarization Assays in Signal Transduction Discovery

Volume: 6 Issue: 3

Author(s): J. Richard Sportsman, Janet Daijo and Elizabeth A. Gaudet

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Keywords: Fluorescence Polarization, adenosine monophosphate, phosphodiesterases

Abstract: Fluorescence polarization (FP) has become widely employed for high throughput screening used in pharmaceutical drug discovery. Assays of important signal transduction targets are now adapted to FP. In this review we examine assays for cyclic adenosine monophosphate, phosphodiesterases, and protein kinases and phosphatases using FP competitive immunoassays and a direct enzymatic method called IMAP.

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Sportsman Richard J., Daijo Janet and Gaudet A. Elizabeth, Fluorescence Polarization Assays in Signal Transduction Discovery, Combinatorial Chemistry & High Throughput Screening 2003; 6 (3) . https://dx.doi.org/10.2174/138620703106298374