iRNA-2methyl: Identify RNA 2'-O-methylation Sites by Incorporating Sequence-Coupled Effects into General PseKNC and Ensemble Classifier

Title:iRNA-2methyl: Identify RNA 2'-O-methylation Sites by Incorporating Sequence-Coupled Effects into General PseKNC and Ensemble Classifier

Volume: 13 Issue: 8

Author(s): Wang-Ren Qiu*, Shi-Yu Jiang , Bi-Qian Sun, Xuan Xiao*, Xiang Cheng and Kuo-Chen Chou*

Affiliation:

• Department of Computer Science and Bond Life Science Center, University of Missouri, Columbia, MO,United States

• Computer Department, Jingdezhen Ceramic Institute, Jingdezhen 333403,China

• Gordon Life Science Institute, Boston, MA 02478,United States

Keywords: 2'-O-Me modification sites, RNA, Sequence-coupled model, Ensemble classifier, Fusion, PseKNC.

Abstract: Objective: Being a kind of post-transcriptional modification (PTCM) in RNA, the 2'-Omethylation modification occurs in the processes of life development and disease formation as well. Accordingly, from the angles of both basic research and drug development, we are facing a challenging problem: given an uncharacterized RNA sequence formed by many nucleotides of A (adenine), C (cytosine), G (guanine), and U (uracil), which one can be of 2-O'-methylation modification, and which one cannot? Unfortunately, so far no computational method whatsoever has been developed to address such a problem.

Method: To fill this empty area, we propose a predictor called iRNA-2methyl. It is formed by incorporating a series of sequence-coupled factors into the general PseKNC (pseudo nucleotide composition), followed by fusing 12 basic random forest classifier into four ensemble predictors, with each aimed to identify the cases of A, C, G, and U along the RNA sequence concerned, respectively.

Results: Rigorous jackknife cross-validations have indicated that the success rates are very high (>93%). For the convenience of most experimental scientists, a user-friendly web-server for iRNA-2methyl has been established at http://www.jci-bioinfo.cn/iRNA-2methyl, by which users can easily obtain their desired results without the need to go through the complicated mathematical equations involved.

Conclusion: The proposed predictor iRNA-2methyl will become a very useful bioinformatics tool for medicinal chemistry, helping to design effective drugs against the diseases related to the 2'-Omethylation modification.

Cite this article as:

Qiu Wang-Ren *, Jiang Shi-Yu , Sun Bi-Qian , Xiao Xuan *, Cheng Xiang and Chou Kuo-Chen*, iRNA-2methyl: Identify RNA 2'-O-methylation Sites by Incorporating Sequence-Coupled Effects into General PseKNC and Ensemble Classifier, Medicinal Chemistry 2017; 13 (8) . https://dx.doi.org/10.2174/1573406413666170623082245