Nanobiocatalysis for the Synthesis of Pentyl Valerate in Organic Solvents: Characterization, Optimization and Reusability Studies

Title:Nanobiocatalysis for the Synthesis of Pentyl Valerate in Organic Solvents: Characterization, Optimization and Reusability Studies

Volume: 7 Issue: 2

Author(s): Vrutika Patel, Milind P. Deshpande, Ashok Pandey, Christian Larroche and Datta Madamwar*

Affiliation:

• Environmental Genomics and Proteomics Lab., PG Department of Biosciences, UGC Centre of Advanced Study, Vadtal Road, Satellite Campus, Sardar Patel University, Bakrol - 388315, Anand, Gujarat,India

Keywords: Biotransformation, Candida rugosa lipase, silanization, enzyme activity, immobilized enzyme, pentyl valerate.

Abstract: Background: Biotransformation and enzymatic methods of ester synthesis are more effective when performed in non-aqueous media. The aim of this work was to prepare reusable and more effective biocatalyst to catalyze the synthesis of pentyl valerate by esterification reaction.

Method: Silane modified CdS nanoparticles were prepared and used for lipase immobilization. Characterization study was carried out using Infrared spectroscopy (FT-IR), Transmission electron microscopy (TEM), Fluorescence microscopy and Thermal gravimetric analysis (TGA). The effects of the reaction parameters such as pH, temperature, and molar ratio of the substrate to the solvent, acid to alcohol chain length was scrutinized in order to get enhanced esterification activity.

Results: Upon optimization, the highest yield obtained for ester synthesis was 86% for immobilized lipase, whereas with free lipase only 52% yield was obtain. The immobilized biocatalyst showed higher catalytic activity (1.6 folds) than crude lipase. Immobilized lipase was recycled for 15 times retaining 80% of its initial activity. K_m and V_max for immobilized lipase are 0.38mM and 205μmoles/mg/min whereas for free lipase K_m was 1.4mM and V_max was 25 µmoles/mg/min.

Conclusion: The application of enzyme immobilized on functionalized CdS verifies to be the promising system for ester synthesis in non-aqueous environment. In addition, nanobioconjugant system proves better for improving catalytic performance of lipase when exploited into organic solvents.

Cite this article as:

Patel Vrutika, Deshpande P. Milind , Pandey Ashok, Larroche Christian and Madamwar Datta*, Nanobiocatalysis for the Synthesis of Pentyl Valerate in Organic Solvents: Characterization, Optimization and Reusability Studies, Current Biotechnology 2018; 7 (2) . https://dx.doi.org/10.2174/2211550106666170530074516