A Combination of Egg Yolk IgY and Phosvitin Inhibits the Growth of Enterotoxigenic Escherichia coli K88 and K99

Title:A Combination of Egg Yolk IgY and Phosvitin Inhibits the Growth of Enterotoxigenic Escherichia coli K88 and K99

Volume: 18 Issue: 5

Author(s): Naiyana Gujral, Heejoo Yoo, Fatemeh Bamdad, Kwang Y. Lee, Joo-Won Suh*Hoon Sunwoo*

Affiliation:

• Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Gyeonggi, 449-728,Korea

• Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 114 St-89 Ave., Edmonton, Alberta,Canada

Keywords: Antimicrobial, egg yolk IgY antibody, Escherichia coli K88, growth inhibition, high hydrostatic pressure, K99, phosvitin.

Abstract: Background: Enterotoxigenic Escherichia coli (ETEC) is the main cause of fatal diarrhea in piglets during the first week of life and over the time of weaning. Pathogenesis of ETEC-causing diarrhea involves intestinal colonization mediated by fimbriae. Although, both IgY and egg yolk phosvitin (PV) possess antimicrobial activity, their combined activity has not been explored. A combination of IgY specific for ETEC and metal-chelating PV may show synergistic effect in reducing the growth of ETEC by inhibiting bacterial proliferation and stipulating protection against ETEC infection.

Objective: The goal of this study was to determine the effects of anti-ETEC IgY and PV on in vitro growth inhibition of ETEC strains possessing K88 and K99 fimbriae prevalent in the porcine population.

Methods: Anti-K88 and -K99 IgY antibodies were obtained from egg yolks of 23-week-old Single- Comb White Leghorn hens immunized with K88 and K99 fimbriae of ETEC, respectively, with high titres sustained over 6 to 8 weeks of the immunization period. Specific IgY, PV, and PV-hydrolysate from alcalase-hydrolysis under high hydrostatic pressure (PVH-Alc-HHP) alone or in combination, were used to treat ETEC K88 and K99 cultures at optimal concentrations of 100 μg/mL, 1 mg/mL, and 1 mg/mL, respectively, for 24 h.

Results: PVH-Alc-HHP demonstrated the highest degree of hydrolysis, 38.9%. Combined use of IgY and PVH-Alc-HHP showed the highest bactericidal effect resulting in ETEC K88 and K99 growth inhibition of 2.8 and 2.67 log CFU/mL, respectively.

Conclusion: Combined IgY-PVH effectively control ETEC, therefore holds a great potential for microbial control in veterinary pharmaceutical industry.

Cite this article as:

Gujral Naiyana, Yoo Heejoo, Bamdad Fatemeh, Lee Y. Kwang, Suh Joo-Won*, Sunwoo Hoon*, A Combination of Egg Yolk IgY and Phosvitin Inhibits the Growth of Enterotoxigenic Escherichia coli K88 and K99, Current Pharmaceutical Biotechnology 2017; 18 (5) . https://dx.doi.org/10.2174/1389201018666170425120036

DOI https://dx.doi.org/10.2174/1389201018666170425120036	Print ISSN 1389-2010
Publisher Name Bentham Science Publisher	Online ISSN 1873-4316