Abstract
Background and Objective: The 4T1 murine breast cancer cell line is commonly employed to study breast cancer metastasis. Transfections to this cell line are frequently carried out using lipid/polymer based gene delivery vehicles. The research paper attempts to use peptide based transfecting agents Str-R8 and MTS-AR8 for in vitro transfection of 4T1 cells.
Methods: These peptides were investigated previously by the authors and showed comparable plasmid DNA complexation abilities. The efficiency of Str-R8, MTS-AR8 in delivery of the plasmid DNA, pSFCMV- SEAP to 4T1 cells is compared to the cationic lipid reagent Lipofectamine®3000. Transfection was measured by estimating the ability of the expressed reporter gene i.e., secreted alkaline phophatase to cleave the substrate para-Nitrophenylphosphate to para-Nitrophenol. Results: Transfection of 4T1 by MTS-AR8/pSF-CMV-SEAP was twofold greater than that mediated by Str-R8/pSF-CMV-SEAP (probability of the values being similar; p<0.05) and comparable to Lipofectamine ®3000/pSF-CMV-SEAP (probability of the values being similar; p>0.5) at a peptide basic amino acid per nucleic acid phosphate ratio of 5:1. Increasing the peptide basic amino acid per nucleic acid phosphate ratio, of MTS-AR8 resulted in a decrease in the transfection of 4T1 cells (probability of the obtained values being similar; p <0.06). Conclusion: The MTS-AR8 peptide has potential and can be further investigated for nucleic acid delivery involving the 4T1 cell line.Keywords: 4T1, MTS-AR8, Str-R8, Lipofectamine, pSF-CMV-SEAP, Transfection.
Graphical Abstract
Drug Delivery Letters
Title:A Cell Penetrating Peptide, MTS-AR8, for Transfection of 4T1 Murine Breast Cancer Cells
Volume: 7 Issue: 1
Author(s): Archana Upadhya and Preeti C. Sangave
Affiliation:
Keywords: 4T1, MTS-AR8, Str-R8, Lipofectamine, pSF-CMV-SEAP, Transfection.
Abstract: Background and Objective: The 4T1 murine breast cancer cell line is commonly employed to study breast cancer metastasis. Transfections to this cell line are frequently carried out using lipid/polymer based gene delivery vehicles. The research paper attempts to use peptide based transfecting agents Str-R8 and MTS-AR8 for in vitro transfection of 4T1 cells.
Methods: These peptides were investigated previously by the authors and showed comparable plasmid DNA complexation abilities. The efficiency of Str-R8, MTS-AR8 in delivery of the plasmid DNA, pSFCMV- SEAP to 4T1 cells is compared to the cationic lipid reagent Lipofectamine®3000. Transfection was measured by estimating the ability of the expressed reporter gene i.e., secreted alkaline phophatase to cleave the substrate para-Nitrophenylphosphate to para-Nitrophenol. Results: Transfection of 4T1 by MTS-AR8/pSF-CMV-SEAP was twofold greater than that mediated by Str-R8/pSF-CMV-SEAP (probability of the values being similar; p<0.05) and comparable to Lipofectamine ®3000/pSF-CMV-SEAP (probability of the values being similar; p>0.5) at a peptide basic amino acid per nucleic acid phosphate ratio of 5:1. Increasing the peptide basic amino acid per nucleic acid phosphate ratio, of MTS-AR8 resulted in a decrease in the transfection of 4T1 cells (probability of the obtained values being similar; p <0.06). Conclusion: The MTS-AR8 peptide has potential and can be further investigated for nucleic acid delivery involving the 4T1 cell line.Export Options
About this article
Cite this article as:
Upadhya Archana and Sangave C. Preeti , A Cell Penetrating Peptide, MTS-AR8, for Transfection of 4T1 Murine Breast Cancer Cells, Drug Delivery Letters 2017; 7 (1) . https://dx.doi.org/10.2174/2210303107666170213101703
DOI https://dx.doi.org/10.2174/2210303107666170213101703 |
Print ISSN 2210-3031 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-304X |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Methods to Access 2-aminobenzimidazoles of Medicinal Importance
Current Organic Chemistry Preclinical Development of Novel Anti-Glioma Drugs Targeting the Endoplasmic Reticulum Stress Response
Current Pharmaceutical Design Proteolytic Cleavage of Notch: “HIT and RUN”
Current Molecular Medicine Patent Selections
Recent Patents on DNA & Gene Sequences Sirtuins: Multifaceted Drug Targets
Current Molecular Medicine Telomere Length Variations in Aging and Age-Related Diseases
Current Aging Science Antiproliferative and Antioxidant Potential of Leaf and Leaf Derived Callus Extracts of Aerva lanata (L.) Juss. Ex Schult. Against Human Breast Cancer (MCF-7) Cell Lines
The Natural Products Journal Repurposing Drugs for Cancer Prevention
Current Topics in Medicinal Chemistry Anticancer Potential of Doxorubicin in Combination with Green-Synthesized Silver Nanoparticle and its Cytotoxicity Effects on Cardio-Myoblast Normal Cells
Anti-Cancer Agents in Medicinal Chemistry Biological Activities, Mechanisms of Action and Biomedical Prospect of the Antitumor Ether Phospholipid ET-18-OCH3 (Edelfosine), A Proapoptotic Agent in Tumor Cells
Current Drug Metabolism miRNAs in Gastrointestinal and Liver Cancers: Their Perspectives and Clinical Applications
Current Pharmaceutical Design Anti-EGFR Strategy for Colorectal Cancer: Searching for the Real Target
Letters in Drug Design & Discovery Effective Inhibition of Mitochondrial Metabolism by Cryptotanshinone in MDA-MB231 cells: A Proteomic Analysis
Current Proteomics PLGA Nanoparticles for Nose to Brain Delivery of Clonazepam: Formulation, Optimization by 32 Factorial Design, In Vitro and In Vivo Evaluation
Current Drug Delivery Dysfunction of Glutamate Receptors in Microglia May Cause Neurodegeneration
Current Alzheimer Research Recent Advances on Liquid Chromatographic and Mass Spectrometric Analysis of Selective Estrogen Receptor Modulators (SERMs) in Biological Fluids
Current Chromatography Intraperitoneal Chemotherapy as First-Line Treatment in the Management of Epithelial Ovarian Cancer
Mini-Reviews in Medicinal Chemistry New Therapies for Prostate Cancer Based on the Biology of Androgen Receptor Coregulators
Current Cancer Therapy Reviews Targeting Indoleamine 2,3-dioxygenase (IDO) to Counteract Tumour- Induced ImmuneDysfunction: From Biochemistry to Clinical Development
Endocrine, Metabolic & Immune Disorders - Drug Targets The Recent Electrochemical Biosensor Technologies for Monitoring of Nucleic Acid Hybridization
Current Analytical Chemistry