Abstract
Peptide drugs have received more attention as potential next-generation drugs or drug leads. Such attention is primarily due to the higher target selectivities and efficiencies of peptide drugs. Moreover, peptide drugs typically have lower accumulation rates and toxicities. Although peptide drugs have advanced significantly in the 21st century, two bottlenecks (disadvantages) primarily hinder peptide drug research and development compared to small molecule drugs. One bottleneck is the identification of a valuable peptide in a complicated pool of crude products; the other bottleneck is the ability to prepare sufficient amounts of peptides for preclinical or clinical trials. This review discusses important methodologies for addressing these two bottlenecks.
Keywords: Extracellular expression, intracellular expression, in vitro screening, in vivo screening, methodology, peptide.
Graphical Abstract
Current Proteomics
Title:The Development of High-throughput Identification and Heterologous Expression of Valuable Peptides/Proteins
Volume: 14 Issue: 1
Author(s): Chang-Jun Liu, Lei Wu, Er Meng and Dong-Yi Zhang
Affiliation:
Keywords: Extracellular expression, intracellular expression, in vitro screening, in vivo screening, methodology, peptide.
Abstract: Peptide drugs have received more attention as potential next-generation drugs or drug leads. Such attention is primarily due to the higher target selectivities and efficiencies of peptide drugs. Moreover, peptide drugs typically have lower accumulation rates and toxicities. Although peptide drugs have advanced significantly in the 21st century, two bottlenecks (disadvantages) primarily hinder peptide drug research and development compared to small molecule drugs. One bottleneck is the identification of a valuable peptide in a complicated pool of crude products; the other bottleneck is the ability to prepare sufficient amounts of peptides for preclinical or clinical trials. This review discusses important methodologies for addressing these two bottlenecks.
Export Options
About this article
Cite this article as:
Liu Chang-Jun, Wu Lei, Meng Er and Zhang Dong-Yi, The Development of High-throughput Identification and Heterologous Expression of Valuable Peptides/Proteins, Current Proteomics 2017; 14 (1) . https://dx.doi.org/10.2174/1570164614666161207162711
DOI https://dx.doi.org/10.2174/1570164614666161207162711 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Hypertension in Pregnancy: Pathophysiology & Management Strategies
Current Pharmaceutical Design Voxel-Based Morphometry of the Human Brain: Methods and Applications
Current Medical Imaging Expression of Signaling Molecules in Progressive Multifocal Leukoencephalopathy
Current HIV Research Crystallization and Preliminary Crystallographic Analysis of Allograft Inflammatory Factor 1
Protein & Peptide Letters Involvement of Membrane Channels in Autoimmune Disorders
Current Pharmaceutical Design Aging and Inflammation: Etiological Culprits of Cancer
Current Aging Science Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes
Current Genomics Endocannabinoids in Neuroimmunology and Stress
Current Drug Targets - CNS & Neurological Disorders The Seattle Structural Genomics Center for Infectious Disease (SSGCID)
Infectious Disorders - Drug Targets New Insights into Chemistry and Anti-Infective Potential of Triazole Scaffold
Current Medicinal Chemistry Characterization of the Lymphotropic Amplicons-6 and Tamplicon-7 Vectors Derived from HHV-6 and HHV-7
Current Gene Therapy Epstein-Barr Encephalitis in a Child with Congenital Human Immunodeficiency Virus Infection: A Case Report Calling for No Forgetfulness
Current HIV Research Neural Stem Cell Niches in Health and Diseases
Current Pharmaceutical Design On the Future Development of Optimally-Sized Lipid-Insoluble Systemic Therapies for CNS Solid Tumors and Other Neuropathologies
Recent Patents on CNS Drug Discovery (Discontinued) NS3 Helicases as Drug Targets
Current Chemical Biology Current Opinions and Perspectives on the Role of Immune System in the Pathogenesis of Parkinson's Disease
Current Pharmaceutical Design EDITORIAL
Current Signal Transduction Therapy Nanoparticle- and Liposome-carried Drugs: New Strategies for Active Targeting and Drug Delivery Across Blood-brain Barrier
Current Drug Metabolism Cytokines in HIV-Infection - Inflammatory Damage or Therapeutic Potential?
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Necroptosis: Who Knew There were so Many Interesting Ways to Die?
CNS & Neurological Disorders - Drug Targets