FTIR Spectroscopy Analysis can Highlight Induced Damage in Neuronallike Cells and Bio-protective Effectiveness of Agmatine

Title:FTIR Spectroscopy Analysis can Highlight Induced Damage in Neuronallike Cells and Bio-protective Effectiveness of Agmatine

Volume: 6 Issue: 1

Author(s): Nadia Ferlazzo, Emanuele Calabro, Monica Curro, Daniela Caccamo, Salvatore Magazu and Riccardo Ientile*

Affiliation:

• Department of Biomedical and Dental Sciences and Morphological and Functional Images, University of Messina, Messina,Italy

Keywords: FTIR, mitochondrial impairment, neuron, oxidative stress, static magnetic fields, scavenging.

Abstract: Background: Agmatine, an endogenous amine, is cosidered a novel neuromodulator with neuroprotective properties. However, the mechanisms involved in these protective effects are poorly understood.

Methods: Fourier Transform Infrared (FTIR) spectroscopy analysis detects biomolecular changes in disordered cells and tissues. In the present study, we employ FTIR spectroscopy to characterize the changes in rotenone-induced damage in neuronal-like differentiated SH-SY5Y neuroblastoma cells in the presence or absence of agmatine.

Results: The analysis of the FTIR spectra evidences significant alterations in rotenone-treated cells that are reduced by the pre-incubation with agmatine (250 nM). In particular, rotenone-damaged cells demonstrate spectral alterations related to amide I, which correspond to an increase in β-sheet components, and decreases in the amide II absorption intensity, suggesting a loss of N-H bending and C-N stretching. These alterations were also evident by Fourier self-deconvolution analysis. Thus, rotenone induced increases in the levels of stretching vibration band related to the protein carboxyl group would account for a significant amount of misfolded proteins in the cell. Agmatine effectively reduces these effects of rotenone on protein structure.

Conclusion: In conclusion, antioxidant and scavenging properties of agmatine reduce rotenoneproduced cellular damage at the level of protein structure. Our results, together with other previous observations, make agmatine a potential therapeutic agent in the treatment of Parkinson's disease.

Cite this article as:

Ferlazzo Nadia, Calabro Emanuele, Curro Monica, Caccamo Daniela, Magazu Salvatore and Ientile Riccardo*, FTIR Spectroscopy Analysis can Highlight Induced Damage in Neuronallike Cells and Bio-protective Effectiveness of Agmatine, Current Metabolomics 2018; 6 (1) . https://dx.doi.org/10.2174/2213235X04666161118144545