Single-Cell Sequencing for Drug Discovery and Drug Development

Title:Single-Cell Sequencing for Drug Discovery and Drug Development

Volume: 17 Issue: 15

Author(s): Hongjin Wu, Charles Wang*Shixiu Wu*

Affiliation:

• Center for Genomics & Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA 92350,United States

• Cancer Research Institute, Hangzhou Cancer Hospital, Hangzhou, 320000, Zhejiang Province,China

Keywords: NGS, Single-cell sequencing, RNA-seq, Drug discovery, Single-cell bisulfite sequencing.

Abstract: Next-generation sequencing (NGS), particularly single-cell sequencing, has revolutionized the scale and scope of genomic and biomedical research. Recent technological advances in NGS and singlecell studies have made the deep whole-genome (DNA-seq), whole-epigenome and whole-transcriptome sequencing (RNA-seq) at single-cell level feasible. NGS at the single-cell level expands our view of genome, epigenome and transcriptome and allows the genome, epigenome and transcriptome of any organism to be explored without a priori assumptions and with unprecedented throughput. And it does so with single-nucleotide resolution. NGS is also a very powerful tool for drug discovery and drug development. In this review, we describe the current state of single-cell sequencing techniques, which can provide a new, more powerful and precise approach for analyzing effects of drugs on treated cells and tissues. Our review discusses single-cell whole genome/exome sequencing (scWGS/scWES), single-cell transcriptome sequencing (scRNA-seq), single-cell bisulfite sequencing (scBS), and multiple omics of single-cell sequencing. We also highlight the advantages and challenges of each of these approaches. Finally, we describe, elaborate and speculate the potential applications of single-cell sequencing for drug discovery and drug development.

Cite this article as:

Wu Hongjin, Wang Charles*, Wu Shixiu*, Single-Cell Sequencing for Drug Discovery and Drug Development, Current Topics in Medicinal Chemistry 2017; 17 (15) . https://dx.doi.org/10.2174/1568026617666161116145358