摘要
针对双链DNA(dsDNA)具有高亲和力和特异性已成为生物化学和分子生物学研究的一个热点。基因诊断与基因治疗、DNA、基因表达的调控可以实现基于一定的识别原理通过特定的相互作用之间的dsDNA和天然核苷酸或合成的配体。一些配体(例如,肽核酸(PNA),三螺旋形成寡核苷酸(TFOs),oligopolyamides,锌指肽)显示足够的亲和力和特异性靶向DNA序列。PNA可以简单的合成和识别DNA序列在生理条件下无限制。本文提供的识别机制、影响因素和审查,PNA靶向DNA识别模式的应用四。这些模式包括三入侵,三联结合,双工,双工入侵,入侵。本文还讨论了未来研究要解决的充分发掘,为特定的DNA识别PNA探针设计的潜在挑战。
关键词: PNA,DNA识别,识别机制、基因诊断、基因治疗。
Current Medicinal Chemistry
Title:Recognition Mechanisms and Applications of Peptide Nucleic Acids Targeting Double-stranded DNA
Volume: 23 Issue: 41
Author(s): Wenjia Li, Huanhuan Shi, Bo Dong, Kaixuan Nie, Zhengchun Liu, Nongyue He
Affiliation:
关键词: PNA,DNA识别,识别机制、基因诊断、基因治疗。
摘要: Targeting double-stranded DNA (dsDNA) with high affinity and specificity has become a hot topic in biochemistry and molecular biology research. Gene diagnosis and therapy, DNA manipulation, and gene expression regulation could be achieved based on certain recognition principles through specific interactions between dsDNA and natural nucleotides or synthesized ligands. Some ligands (e.g., peptide nucleic acids (PNAs), triple helix-forming oligonucleotides (TFOs), oligopolyamides, and zinc-finger peptides) show sufficient affinity and specificity for targeting dsDNA sequences. PNA can simply synthesize and recognize dsDNA without sequence limitation under physiological conditions. This paper provides a review on the recognition mechanisms, influencing factors, and applications of the four recognition modes of PNA targeting dsDNA. These modes include triplex invasion, triplex binding, duplex invasion, and double duplex invasion. This paper also discusses the challenges to be addressed by future research to fully explore the potential of PNA probe design for specific dsDNA recognition.
Export Options
About this article
Cite this article as:
Wenjia Li, Huanhuan Shi, Bo Dong, Kaixuan Nie, Zhengchun Liu, Nongyue He , Recognition Mechanisms and Applications of Peptide Nucleic Acids Targeting Double-stranded DNA, Current Medicinal Chemistry 2016; 23 (41) . https://dx.doi.org/10.2174/0929867323666161028154243
DOI https://dx.doi.org/10.2174/0929867323666161028154243 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements