Abstract
Background: Biosurfactants are biological origin surfactants, produced by microorganisms and reduce surface and interfacial tension between liquids and solids. They have many outstanding properties, such as lower toxicity, biodegradability, selectivity and specific activity at extreme conditions, compared with synthetic ones. Despite all these positive features high production cost prevents the widespread use of biosurfactants as surface active substances. So using low cost material such as wastes, is important for reducing high cost of production.
Objective: The aim of this study was to isolate highly productive biosurfactant producer Pseudomonas spp. strains and ramp up rhamnolipid production. Method: Pseudomonas putida IBS036 and Pseudomonas pachastrellae LOS20 strains isolated from environmental soil samples as well as Pseudomonas aeruginosa ATCC 9027 strain were also included in the study to ramp up rhamnolipid production. For this purpose, different media were formed using pulps of barley, hazelnut and sunflower. The rhamnolipid amounts were determined with UV spectrophotometer. Result: As a result of present work, the best media for rhamnolipid production were determined as barley pulp enhanced with glycerol (9.3 g L-1) and hazelnut (11.1 g L-1) for P. aeruginosa ATCC 9027, barley pulp (9.2 g L-1) for P. pachastrellae and hazelnut (8.5 g L-1) and sunflower (6.7 g L-1) for P. putida. Conclusion: Finally, the results confirmed the potential usage of these alternative substrates for production of rhamnolipid.Keywords: Biosurfactant, barley, hazelnut, sunflower, pulp, Pseudomonas pachastrellae.
Graphical Abstract
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