Methods to Evaluate the Activity of Nitric Oxide Synthase

Title:Methods to Evaluate the Activity of Nitric Oxide Synthase

Volume: 13 Issue: 5

Author(s): Marialuigia Fantacuzzi*, Alessandra Ammazzalorso, Barbara De Filippis, Letizia Giampietro, Cristina Maccallini and Rosa Amoroso

Affiliation:

• Department of Pharmacy, “G. d’Annunzio” University, Chieti,Italy

Keywords: Nitric oxide synthase, nitric oxide, L-arginine, L-citrulline, griess assay, hemoglobin assay, citrulline assay, RPHPLC assay.

Abstract: Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented.

Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay.

Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of Lcitrulline as stoichiometric to nitric oxide.

Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.

Cite this article as:

Fantacuzzi Marialuigia*, Ammazzalorso Alessandra, De Filippis Barbara, Giampietro Letizia, Maccallini Cristina and Amoroso Rosa, Methods to Evaluate the Activity of Nitric Oxide Synthase, Current Pharmaceutical Analysis 2017; 13 (5) . https://dx.doi.org/10.2174/1573412912666160916154405

DOI https://dx.doi.org/10.2174/1573412912666160916154405	Print ISSN 1573-4129
Publisher Name Bentham Science Publisher	Online ISSN 1875-676X