Elucidation of Phosphodiesterase-1 Inhibitory Effect of Some Selected Natural Polyphenolics Using In Vitro and In Silico Methods

Title:Elucidation of Phosphodiesterase-1 Inhibitory Effect of Some Selected Natural Polyphenolics Using In Vitro and In Silico Methods

Volume: 17 Issue: 4

Author(s): Abdur Rauf, Ilkay Erdogan Orhan, Abdulselam Ertas, Hamdi Temel, Taibi Ben Hadda, Muhammad Saleem, Muslim Raza and Haroon Khan

Affiliation:

Keywords: Polyphenolics, Phosphodiesterase, Enzyme inhibition, In silico, Chlorogenic acid.

Abstract: Background: Polyphenols are organic compounds widely found in plants and possess many diverse biological activities. In the current study, eleven polyphenolic compounds [rutin (1), trans-ferulic acid (2), epigallaocatechin gallate (3), fumaric acid (4), chlorogenic acid (5), gallic acid (6), trans-resveratrol (7), propyl gallate (8), (+)-catechin (9), quercetin (10), and kaempferol-3-O-β-D-galactoside (11)] have been evaluated for their in vitro inhibitory activity against phosphodiesterase- 1 (PDE-1). The active compounds 1, 5, 6, and 7 possessed IC₅₀ values of 173.90 ± 2.58, 36.67 ± 1.60, 325.19 ± 3.62, and 323.81 ± 4.25 μM, respectively. In silico experiments were performed to elucidate the binding patterns of inhibitor molecules with the active sites of the enzymes.

Cite this article as:

Rauf Abdur, Orhan Erdogan Ilkay, Ertas Abdulselam, Temel Hamdi, Hadda Ben Taibi, Saleem Muhammad, Raza Muslim and Khan Haroon, Elucidation of Phosphodiesterase-1 Inhibitory Effect of Some Selected Natural Polyphenolics Using In Vitro and In Silico Methods, Current Topics in Medicinal Chemistry 2017; 17 (4) . https://dx.doi.org/10.2174/1568026616666160824103615