Abstract
Background: Nano biosensors are highly sensitive and cost effective nanostructured metal oxides because of their excellent properties such as optical, electrical, selectivity and surface to volume ratio. Apart from these properties can also possess other unique properties like biocompatibility, nontoxic, high iso electric point (iep) (~9.2). Now most of the researchers are attracted towards core-shell nps, since these fabrics have emerged in many areas such as fluorescence imaging.
Method: Synthesis of ceria-titania (CeO2-TiO2) Core-Shell Nano Particles (CSNPs) from the wet chemical precipitation method using cerium-nitrate hexa-hydrate and Titanium tetra isopropoxide (TTIP) as precursors.
Results: Chemically synthesized biocompatible ceria-titania (CeO2-TiO2) CSNPs were characterized by High resolution transmission electron microscopy (HRTEM), Scanning electron microscopy (SEM), UV-Visible spectroscopy and Cyclic Voltammetry (CV) techniques. A novel glucose biosensor based ceria-titania CSNPs was developed by immobilizing glucose 6 phosphate dehydrogenase (G6PD) with nicotinamide adenine dinucleotide phosphate (NADPH) on CSNPs via covalent linkage.
Conclusion: The glucose sensing performance of an electrochemical biosensor with nano-interface was successfully investigated by fabricating NADPH/G6PD/TiO2 coated CeO2 core shell NPs/Pt bioelectrode. The developed biosensor exhibited high sensitivity (2.11 V mM-1), Limit of detection (2.98μM) and with a linear dynamic range of 0.5-2.5mM. As the detection limit is very low, this sensor can detect glucose from this lower limit. In addition, the normal range of blood glucose is about 3.9- 7.8mM. Owing to its high response, the fabricated TiO2 coated CeO2 core shell nano-interface based bio-electrode can be used as a sensitive handheld miniaturize system for the detection of glucose in blood samples.
Keywords: CeO2-TiO2 core shell nanoparticles, wet chemical method, glucose bio sensor, glucose 6 phosphate dehydrogenase, nicotinamide adenine dinucleotide phosphate, cyclic Voltammetry.
Graphical Abstract
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