Synthesis, Molecular Docking Study and in vitro Anticancer Activity of Tetrazole Linked Benzochromene Derivatives

Title:Synthesis, Molecular Docking Study and in vitro Anticancer Activity of Tetrazole Linked Benzochromene Derivatives

Volume: 17 Issue: 3

Author(s): Sridevi Gorle, Suresh Maddila, Surya N. Maddila, Kovashnee Naicker, Moganavelli Singh, Parvesh Singh and Sreekantha B. Jonnalagadda

Affiliation:

Keywords: Synthesis, tetrazoles, benzochromenes, cytotoxicity, docking study, ATP binding.

Abstract: Background: Globally, cancer is regarded as one of the biggest health concern in humans and animals and is one of the most terrifying diseases. Therefore, there is a necessity for the discovery, development and improvement of novel antitumor drug molecules which could efficiently prevent proliferative pathways and clonal expansion of cells. Heterocyclic compounds like benzochromene play a key role in the development of current pharmaceuticals, natural resources, agriculture products, analytical reagents and dyes. Therefore, anticancer drugs show increased resistance, it is essential to designing the novel structured heterocyclic moieties to create potential anticancer agents with promising biological applications.

Objective: To synthesis a novel 1-(substitutedphenyl)-2-(1H-tetrazol-5-yl)-1H-benzo[f]chromene-3-amine derivatives for in vitro antitumour activity.

Method: The reaction of 3-amino-1-(substitutedphenyl)-1H-benzo[f]chromene-2-carbonitrile with sodium azide, ammonium chloride in dimethyl formamide solvent under reflux condition for 4 h afforded products (3a-k). The synthesized molecules were subjected to possible potential anti-tumour activity in vitro in four human cancer cell lines (MCF-7, Caco-2, HeLa and SKBR-3), and one human non-cancer cell line (HEK293), using the MTT cell viability assay.

Results: A novel series of products (3a-k) were synthesized with good yield and were identified with ¹H NMR, ¹⁵N NMR, ¹³C NMR, FT-IR and HR-MS spectrum. The most potent compounds 3d, 3e, and 3f possessing the greatest cytotoxicity activity with IC₅₀ values slightly higher (15-33 μM) than that of 5-Fluorouracil (10-17 μM), indicating their potential to be antitumor agents. The 3a, 3b, 3c, 3h, 3i and 3j compounds showed moderate activity. Additionally, a molecular docking analysis was conducted to predict the multi-drug resistance modulator behavior of synthesized compounds in the ATP binding site of P-glycoprotein.

Conclusions: We synthesized and designated eleven novel derivatives of tetrazole linked benzochromenes (3a-k) and evaluated their anti-cancer activity. Additionally, the results from the docking studies were found to be in good agreement with the results from computational profiling.

Cite this article as:

Gorle Sridevi, Maddila Suresh, Maddila N. Surya, Naicker Kovashnee, Singh Moganavelli, Singh Parvesh and Jonnalagadda B. Sreekantha, Synthesis, Molecular Docking Study and in vitro Anticancer Activity of Tetrazole Linked Benzochromene Derivatives, Anti-Cancer Agents in Medicinal Chemistry 2017; 17 (3) . https://dx.doi.org/10.2174/1871520616666160627090249

DOI https://dx.doi.org/10.2174/1871520616666160627090249	Print ISSN 1871-5206
Publisher Name Bentham Science Publisher	Online ISSN 1875-5992