Distribution of Podoplanin in Synovial Tissues in Rheumatoid Arthritis Patients Using Biologic or Conventional Disease-Modifying Anti-Rheumatic Drugs

Title:Distribution of Podoplanin in Synovial Tissues in Rheumatoid Arthritis Patients Using Biologic or Conventional Disease-Modifying Anti-Rheumatic Drugs

Volume: 13 Issue: 1

Author(s): Yuya Takakubo, Hiroharu Oki, Yasushi Naganuma, Kan Saski, Akiko Sasaki, Yasunobu Tamaki, Yang Suran, Tsuneo Konta and Michiaki Takagi

Affiliation:

Keywords: Anti-rheumatic drugs, invasion, podoplanin, Podoplanin, rheumatoid arthritis, synovial tissues.

Abstract: Objective: Podoplanin (PDPN) mediates tumor cell migration and invasion, which phenomena might also play a role in severe rheumatoid arthritis (RA). Therefore, the precise cellular distribution of PDPN and it’s relationships with inflammation was studied in RA treated with biologic disease-modifying anti-rheumatic drugs (DMARD) or conventional DMARDs (cDMARD).

Methods: PDPN+ cells were immunostained by NZ-1 mAb, and scored (3+; >50%/ area, 2+; 20%- 50%, 1+; 5%-20%, 0: <5%) in synovial tissues from RA treated with biologic DMARDs (BIO, n=20) or cDMARD (n=20) for comparison with osteoarthritis (OA, n=5), followed by cell grading of inflammation and cell-typing.

Results: Inflammatory synovitis score was 1.4 in both BIO and cDMARD, compared to only 0.2 in OA. PDPN+ cells were found in the lining layer (BIO 1.6, cDMARD 1.3, OA 0.2) and lymphoid aggregates (BIO 0.6, cDMRD 0.7, OA 0.2), and correlated with RA-inflammation in BIO- and cDMARD-groups in both area (r=0.7/0.9, r=0.6/0.7, respectively p<0.05). PDPN was expressed in CD68+ type A macrophage-like and 5B5+ type B fibroblast-like cells in the lining layer, and in IL- 17+ cells in lymphoid aggregates in RA.

Conclusion: PDPN was markedly increased in the immunologically inflamed RA synovitis, which was surgically treated due to BIO- and cDMARD-resistant RA. PDPN may have potential of a new marker of residual arthritis in local joints for inflammation-associated severe RA.

Cite this article as:

Takakubo Yuya, Oki Hiroharu, Naganuma Yasushi, Saski Kan, Sasaki Akiko, Tamaki Yasunobu, Suran Yang, Konta Tsuneo and Takagi Michiaki, Distribution of Podoplanin in Synovial Tissues in Rheumatoid Arthritis Patients Using Biologic or Conventional Disease-Modifying Anti-Rheumatic Drugs, Current Rheumatology Reviews 2017; 13 (1) . https://dx.doi.org/10.2174/1573397112666160331143607

DOI https://dx.doi.org/10.2174/1573397112666160331143607	Print ISSN 1573-3971
Publisher Name Bentham Science Publisher	Online ISSN 1875-6360