在子宫内膜异位症中10-11转基因的下调

Title:Ten-Eleven Translocation Genes are Downregulated in Endometriosis

Volume: 16 Issue: 3

Author(s): F.J. Roca, H.A. Loomans, A.T. Wittman, C.J. Creighton and S.M. Hawkins

Affiliation:

关键词: 5-羟甲基胞嘧啶（5-hmC），子宫内膜异位症，表观遗传修饰，母细胞（子宫内膜间质成纤维细胞），在体外蜕膜化，TET（10-11转）基因。

摘要: Our previous whole genome expression analysis of endometriomas suggested dysregulation of the ten-eleven translocation genes (TET1, TET2, and TET3), involved in converting 5- methylcytosine to 5-hydroxymethylcytosine (5-hmC). The objective of this study was to validate the expression of TET genes in ectopic and eutopic endometrium and in primary cultures of human endometrial stromal fibroblasts (HESF) during in vitro decidualization and to quantify 5-hmC levels in patients with endometriosis. Blood, eutopic endometrium, and endometriotic tissues were collected at time of gynecologic surgery. HESF cultures were created from eutopic endometrium of women without (HESF-CONTROL) and with endometriosis (HESF-ENDO) and underwent in vitro decidualization. Genomic DNA from blood and tissues underwent quantification of the absolute amount of 5-hmC using ELISA. The expression of TET1, TET2, and TET3 was decreased in endometriosis compared to non-endometriosis control eutopic endometrium. Surprisingly, the global amount of 5-hmC was higher in ectopic endometrium than control eutopic endometrium, while genomic DNA from blood of women with endometriosis contained statistically significantly less 5-hmC than women without endometriosis. Expression of TET1, TET2, and TET3 was decreased in non-decidualized HESFENDO. Upon in vitro decidualization, control HESF showed decreased expression of TET3, while decidualized HESF-ENDO showed no statistically significant change in expression of TET1, TET2, or TET3. These results indicate that the TET genes are downregulated in ectopic endometrium and in HESF-ENDO, and suggest for the first time that TET genes play a role in endometriosis. High global amounts of 5-hmC in endometriotic tissues suggest unique epigenetic regulation in these tissues.

Cite this article as:

F.J. Roca, H.A. Loomans, A.T. Wittman, C.J. Creighton and S.M. Hawkins , Ten-Eleven Translocation Genes are Downregulated in Endometriosis, Current Molecular Medicine 2016; 16 (3) . https://dx.doi.org/10.2174/1566524016666160225153844