Biosynthesis of Fluorescent Bi₂S₃ Nanoparticles and their Application as Dual-Function SPECT-CT Probe for Animal Imaging

Title:Biosynthesis of Fluorescent Bi₂S₃ Nanoparticles and their Application as Dual-Function SPECT-CT Probe for Animal Imaging

Volume: 16 Issue: 18

Author(s): Imran Uddin, Absar Ahmad, Ejaz Ahmad Siddiqui, Sk. Hasanur Rahaman and Sanjay Gambhir

Affiliation:

Keywords: Biosynthesis, Bismuth sulphide, Characterization, Fungus, Nanoparticles, SPECT-CT.

Abstract: Bismuth sulphide (Bi₂S₃) is an excellent semiconductor and its nanoparticles have numerous significant applications including photovoltaic materials, photodiode arrays, bio-imaging, etc. Nevertheless, these nanoparticles when fabricated by chemical and physical routes tend to easily aggregate in colloidal solutions, are eco-unfriendly, cumbrous and very broad in size distribution. The aim of the present manuscript was to ecologically fabricate water dispersible, safe and stable Bi₂S₃ nanoparticles such that these may find use in animal imaging, diagnostics, cell labeling and other biomedical applications. Herein, we for the first time have biosynthesized highly fluorescent, natural protein capped Bi₂S₃ nanoparticles by subjecting the fungus Fusarium oxysporum to bismuth nitrate pentahydrate [Bi(NO₃)₃.5H₂O] alongwith sodium sulphite (Na₂SO₃) as precursor salts under ambient conditions of temperature, pressure and pH. The nanoparticles were completely characterized using recognized standard techniques. These natural protein capped Bi2S3 nanoparticles are quasi-spherical in shape with an average particle size of 15 nm, maintain long term stability and show semiconductor behavior having blue shift with a band gap of 3.04 eV. Semiconductor nanocrystals are fundamentally much more fluorescent than the toxic fluorescent chemical compounds (fluorophores) which are presently largely employed in imaging, immunohistochemistry, biochemistry, etc. Biologically fabricated fluorescent nanoparticles may replace organic fluorophores and aid in rapid development of biomedical nanotechnology. Thus, biodistribution study of the so-formed Bi₂S₃ nanoparticles in male Sprague Dawley rats was done by radiolabelling with Technitium-99m (Tc-99m) and clearance time from blood was calculated. The nanoparticles were then employed in SPECT-CT probe for animal imaging where these imparted iodine equivalent contrast.

Cite this article as:

Uddin Imran, Ahmad Absar, Ahmad Siddiqui Ejaz, Hasanur Rahaman Sk. and Gambhir Sanjay, Biosynthesis of Fluorescent Bi₂S₃ Nanoparticles and their Application as Dual-Function SPECT-CT Probe for Animal Imaging, Current Topics in Medicinal Chemistry 2016; 16 (18) . https://dx.doi.org/10.2174/1568026616666160215155347