UV-Visible Absorption Spectroscopy Enhanced X-ray Crystallography at Synchrotron and X-ray Free Electron Laser Sources

Title:UV-Visible Absorption Spectroscopy Enhanced X-ray Crystallography at Synchrotron and X-ray Free Electron Laser Sources

Volume: 23 Issue: 3

Author(s): Aina E. Cohen, Tzanko Doukov and Michael S. Soltis

Affiliation:

Keywords: Crystallography, radiation damage, synchrotron, UV-visible absorption spectroscopy, XFEL.

Abstract: This review describes the use of single crystal UV-Visible Absorption micro-Spectrophotometry (UV-Vis AS) to enhance the design and execution of X-ray crystallography experiments for structural investigations of reaction intermediates of redox active and photosensitive proteins. Considerations for UV-Vis AS measurements at the synchrotron and associated instrumentation are described. UV-Vis AS is useful to verify the intermediate state of an enzyme and to monitor the progression of reactions within crystals. Radiation induced redox changes within protein crystals may be monitored to devise effective diffraction data collection strategies. An overview of the specific effects of radiation damage on macromolecular crystals is presented along with data collection strategies that minimize these effects by combining data from multiple crystals used at the synchrotron and with the X-ray free electron laser.

Cite this article as:

Cohen E. Aina, Doukov Tzanko and Soltis S. Michael, UV-Visible Absorption Spectroscopy Enhanced X-ray Crystallography at Synchrotron and X-ray Free Electron Laser Sources, Protein & Peptide Letters 2016; 23 (3) . https://dx.doi.org/10.2174/0929866523666160107115015

DOI https://dx.doi.org/10.2174/0929866523666160107115015	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305