Abstract
In order to examine underlying mechanisms of the impressive regeneration in Dendrorhynchus Zhejiangensis, a two-dimensional gel electrophoresis (2-DE) combined with mass spectrometric analysis was employed. 70 proteins involved in regenerative process were identified and 33 proteins were up-regulated and others were down-regulated in fracture injured model. Among them, about 79% were cell cytoskeleton proteins and motor proteins. As the representatives, the expression profiles of Actin and Gelsolin-like protein (GSN), both of which were involved in cell motility, in response to body breakage of nemerteans were examined. Two genes showed similar expression patterns during regeneration process and both of them were first constantly up-regulated at 1h and 3h after being broken, then down-regulated to a lower level at 3rd day. The results suggested that Actin and GSN play important roles in regeneration of D. Zhejiangensis.
Keywords: Two-dimensional gel electrophoresis, gelsolin-like protein, actin; nemertean, regeneration, expression.
Graphical Abstract
Current Proteomics
Title:Identification of Differentially Expressed Proteins During Regeneration of Dendrorhynchus zhejiangensis Using the Comparative Proteomic Analysis
Volume: 12 Issue: 3
Author(s): Ye Li, Lei Chen, Jun Zhou, Xiurong Su and Chundan Zhang
Affiliation:
Keywords: Two-dimensional gel electrophoresis, gelsolin-like protein, actin; nemertean, regeneration, expression.
Abstract: In order to examine underlying mechanisms of the impressive regeneration in Dendrorhynchus Zhejiangensis, a two-dimensional gel electrophoresis (2-DE) combined with mass spectrometric analysis was employed. 70 proteins involved in regenerative process were identified and 33 proteins were up-regulated and others were down-regulated in fracture injured model. Among them, about 79% were cell cytoskeleton proteins and motor proteins. As the representatives, the expression profiles of Actin and Gelsolin-like protein (GSN), both of which were involved in cell motility, in response to body breakage of nemerteans were examined. Two genes showed similar expression patterns during regeneration process and both of them were first constantly up-regulated at 1h and 3h after being broken, then down-regulated to a lower level at 3rd day. The results suggested that Actin and GSN play important roles in regeneration of D. Zhejiangensis.
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Cite this article as:
Li Ye, Chen Lei, Zhou Jun, Su Xiurong and Zhang Chundan, Identification of Differentially Expressed Proteins During Regeneration of Dendrorhynchus zhejiangensis Using the Comparative Proteomic Analysis, Current Proteomics 2015; 12 (3) . https://dx.doi.org/10.2174/157016461203151120094027
DOI https://dx.doi.org/10.2174/157016461203151120094027 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
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