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Current Pharmaceutical Biotechnology

Editor-in-Chief

ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Towards Sorting of Biolibraries Using Single-Molecule Fluorescence Detection Techniques

Author(s): Antonie J.W.G. Visser, Beno H. Kunst, Hans Keller and Arjen Schots

Volume 5, Issue 2, 2004

Page: [173 - 179] Pages: 7

DOI: 10.2174/1389201043376977

Price: $65

Abstract

The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation of biolibraries, single-molecule detection techniques and microfluidic devices. A highthroughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits.

Keywords: biolibrary, phage display, selection, sorting


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