Abstract
The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation of biolibraries, single-molecule detection techniques and microfluidic devices. A highthroughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits.
Keywords: biolibrary, phage display, selection, sorting
Current Pharmaceutical Biotechnology
Title: Towards Sorting of Biolibraries Using Single-Molecule Fluorescence Detection Techniques
Volume: 5 Issue: 2
Author(s): Antonie J.W.G. Visser, Beno H. Kunst, Hans Keller and Arjen Schots
Affiliation:
Keywords: biolibrary, phage display, selection, sorting
Abstract: The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation of biolibraries, single-molecule detection techniques and microfluidic devices. A highthroughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits.
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Cite this article as:
Visser J.W.G. Antonie, Kunst H. Beno, Keller Hans and Schots Arjen, Towards Sorting of Biolibraries Using Single-Molecule Fluorescence Detection Techniques, Current Pharmaceutical Biotechnology 2004; 5 (2) . https://dx.doi.org/10.2174/1389201043376977
DOI https://dx.doi.org/10.2174/1389201043376977 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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