Art and Artefacts of Fluorescence Correlation Spectroscopy

Title: Art and Artefacts of Fluorescence Correlation Spectroscopy

Volume: 5 Issue: 2

Author(s): Jorg Enderlein, Ingo Gregor, Digambara Patra and Jorg Fitter

Affiliation:

Keywords: fluorescence correlation spectroscopy, diffusion coefficients, confocal detection

Abstract: Fluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient of the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.

Cite this article as:

Enderlein Jorg, Gregor Ingo, Patra Digambara and Fitter Jorg, Art and Artefacts of Fluorescence Correlation Spectroscopy, Current Pharmaceutical Biotechnology 2004; 5 (2) . https://dx.doi.org/10.2174/1389201043377020