Alterations of the X Chromosome in Lymphocytes of Alzheimer’s Disease Patients

Title:Alterations of the X Chromosome in Lymphocytes of Alzheimer’s Disease Patients

Volume: 12 Issue: 10

Author(s): Biljana Spremo-Potparevic, Vladan Bajic, George Perry and Lada Zivkovic

Affiliation:

Keywords: Alzheimer’s disease, aneuploidy, FISH, peripheral blood lymphocytes, PCD, X chromosome.

Abstract: Chromosomal alterations as a sign of genetic instability are a feature of Alzheimer’s disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p<0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p<0.001). In addition, a strong (R2=0.97, n=20) and significant (p<0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.

Cite this article as:

Spremo-Potparevic Biljana, Bajic Vladan, Perry George and Zivkovic Lada, Alterations of the X Chromosome in Lymphocytes of Alzheimer’s Disease Patients, Current Alzheimer Research 2015; 12 (10) . https://dx.doi.org/10.2174/1567205012666151027124154