Improving Properties of Recombinant SsoPox by Site-Specific Pegylation

Title:Improving Properties of Recombinant SsoPox by Site-Specific Pegylation

Volume: 22 Issue: 12

Author(s): Harsh Parikh, Priyanka Bajaj, Rajan K. Tripathy and Abhay H. Pande

Affiliation:

Keywords: rSsoPox, mono-PEGylation, organophosphate, protease digestion, thermostability.

Abstract: SsoPox, a ~35 kDa enzyme from Sulfolobus solfataricus, can hydrolyze and inactivate a variety of organophosphate (OP)-compounds. The enzyme is a potential candidate for the development of prophylactic and therapeutic agent against OP-poisoning in humans. However, the therapeutic use of recombinant SsoPox suffers from certain limitations associated with the use of recombinant protein pharmaceuticals. Some of these limitations could be overcome by conjugating SsoPox enzyme with polyethylene glycol (PEG). In this study, we report generation and in vitro characterization of N-terminal mono-PEGylated rSsoPox_(2p) (a variant of rSsoPox_(wt) having enhanced OP-hydrolyzing activity). The enzyme was PEGylated with mPEG-propionaldehyde and the PEGylated protein was isolated using ion-exchange chromatography. Compared with the unmodified enzyme, mono-PEGylation of rSsoPox results in improvement in the thermostability and protease resistance of the enzyme. PEGylated rSsoPox_(2p) can be developed as a candidate for the prevention / treatment of OP-poisoning.

Cite this article as:

Parikh Harsh, Bajaj Priyanka, Tripathy K. Rajan and Pande H. Abhay, Improving Properties of Recombinant SsoPox by Site-Specific Pegylation, Protein & Peptide Letters 2015; 22 (12) . https://dx.doi.org/10.2174/0929866522666151002122751

DOI https://dx.doi.org/10.2174/0929866522666151002122751	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305