小白蛋白和MiR-181a对鳜鱼快速肌松的调节作用

Title:Rapid Muscle Relaxation in Siniperca chuatsi is Coordinated by Parvalbumin (PVALB) and MiR-181a

Volume: 15 Issue: 8

Author(s): W. -Y. Chu, L. -S. Liu, L. Chen, P. -H. Yang, Y. -L. Li, Y. -H. Wang and J. -H. Wang, P. Wu, X. Zhu and J. -S. Zhang

Affiliation:

关键词: miR-181a，肌肉松弛，小白蛋白，调节，鳜鱼。

摘要: Parvalbumins (PVALBs) are particularly abundant in the fast-contracting muscles and correlate positively with muscle relaxation speed in amphibians and fishes. MiRNAs play important roles in diverse biologic processes via binding to the 3’ untranslated region (3’UTR) of the target mRNAs. In the study, four PVALB isoforms, named as PVALB1, 2, 3, and 4, were identified in the mandarin fish (Siniperca chuatsi) fast muscle and PVALB4 exhibited the highest expression level among them. By bioinformatics analysis, a putative miR-181a binding site in PVALB4 was detected and the direct interaction between miR-181a and PVALB4 was confirmed with the luciferase reporter assay. Further, when miR-181a was inhibited, it substantially increased PVALB4 mRNA expression level and the muscle relaxation rate in vivo. Taken together, the obtained results suggest that miRNA-181a/PVALB4 is an evolutionarily conserved miRNAtarget pair and their interaction is correlated with muscle relaxation rate in the mandarin fish. Therefore, the study revealed a novel molecular mechanism in the regulation of skeletal muscle relaxation in fish.

Cite this article as:

W. -Y. Chu, L. -S. Liu, L. Chen, P. -H. Yang, Y. -L. Li, Y. -H. Wang and J. -H. Wang, P. Wu, X. Zhu and J. -S. Zhang , Rapid Muscle Relaxation in Siniperca chuatsi is Coordinated by Parvalbumin (PVALB) and MiR-181a, Current Molecular Medicine 2015; 15 (8) . https://dx.doi.org/10.2174/1566524015666150921110037