Abstract
Kinnow mandarin (Citrus reticulata blanco) is the popular fruit crop in the world. In this study 12.5 % sodium dodecyl sulphate polyacryl amide gel electrophoresis (SDS-PAGE) method was used to observe comparative protein production pattern in different tissue culture stages of Kinnow mandarin. In this study embryogenic calli, non embryogenic calli, nucellar embryo, in vitro grown plant and seeds were used. In SDS gel total 31 protein bands were observed among them 9 or (29%) bands were polymorphic and 22 or (79.96%) were monomorphic with molecular weight ranging from 26 to 170 KDa of size. Two protein bands were similar among all tissue culture stages one was of 180 KDa and the other one between 72-55 KDa. Based on their banding pattern, similarity matrix with Jaccard’s coefficients was calculated and found 0.31 to 0.80. Dendrogram was constructed with Unweighted Pair Group Method with Arithmetic averages (UPGMA) which separated all bands into two main clusters. This comprised of seed and non embryogenic callus in one cluster and embryogenic callus, nucellar embryo in one group and in cluster with in vitro plant, respectively. It concludes that embryogenic callus and nucellar embryos have 80% similarity in their protein production profile, suggesting their similar properties for regeneration as both stages possess regenerative potential.
Keywords: Embryogenic calli, in vitro grown plant, non embryogenic calli, nucellar embryo, protein profiling, seeds.
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