Abstract
Toxicogenomics is an emerging technology that defines the use of novel genomic techniques to investigate the adverse effects of xenobiotic on gene expression. Toxicogenomics is based on the fact that most of relevant toxicological effects of a compound affect directly or indirectly the gene expression. The most common methods to profile gene expression at the transcript level are Northern Blotting and the real-time PCR. While commonly used and well accepted, these techniques are now superseded by new technologies allowing the analysis of the expression for multiple genes simultaneously. DNA microarrays are now developed for simultaneous gene analysis but inherent to such multiple assays, their quantitative aspect and their relevance for toxicogenomics have been questioned. We will review here recent studies on their use for toxicogenomics and examine the possible future of such technology in complementation with the other toxicology methods.
Keywords: low-density microarray, quantitative assay, mechanistic toxicogenomics, drug metabolism, predictive toxicogenomics, rat liver
Combinatorial Chemistry & High Throughput Screening
Title: DNA Microarrays as a Tool in Toxicogenomics
Volume: 7 Issue: 3
Author(s): F. de Longueville, V. Bertholet and J. Remacle
Affiliation:
Keywords: low-density microarray, quantitative assay, mechanistic toxicogenomics, drug metabolism, predictive toxicogenomics, rat liver
Abstract: Toxicogenomics is an emerging technology that defines the use of novel genomic techniques to investigate the adverse effects of xenobiotic on gene expression. Toxicogenomics is based on the fact that most of relevant toxicological effects of a compound affect directly or indirectly the gene expression. The most common methods to profile gene expression at the transcript level are Northern Blotting and the real-time PCR. While commonly used and well accepted, these techniques are now superseded by new technologies allowing the analysis of the expression for multiple genes simultaneously. DNA microarrays are now developed for simultaneous gene analysis but inherent to such multiple assays, their quantitative aspect and their relevance for toxicogenomics have been questioned. We will review here recent studies on their use for toxicogenomics and examine the possible future of such technology in complementation with the other toxicology methods.
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Cite this article as:
Longueville de F., Bertholet V. and Remacle J., DNA Microarrays as a Tool in Toxicogenomics, Combinatorial Chemistry & High Throughput Screening 2004; 7 (3) . https://dx.doi.org/10.2174/1386207043328841
DOI https://dx.doi.org/10.2174/1386207043328841 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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