A New Phospholipase A₂ from Lachesis muta rhombeata: Purification, Biochemical and Comparative Characterization with Crotoxin B

Title:A New Phospholipase A₂ from Lachesis muta rhombeata: Purification, Biochemical and Comparative Characterization with Crotoxin B

Volume: 22 Issue: 9

Author(s): Francielle A. Cordeiro, Tibério G.K. Perini, Cristiane Bregge-Silva, Caroline M. Cremonez, Renata S. Rodrigues, Johara Boldrini-França, Karla de C.F. Bordon, Dayane L.N. De Souza, David C. Ache, Veridiana de M. Rodrigues, Wagner F. dos Santos, Jose C. Rosa and Eliane C. Arantesa

Affiliation:

Keywords: Acidic phospholipase A2, crotoxin B, edema, Lachesis muta rhombeata, myotoxicity, snake venom.

Abstract: Phospholipases A₂ (PLA₂s) are enzymes responsible for inflammatory effects, edema formation, myotoxicity, neurotoxicity and other manifestations from envenoming. In this paper we report the isolation and biochemical characterization of Lmr-PLA2, the first acidic PLA₂ found in Lachesis muta rhombeata venom. Furthermore, this study compared biological effects of Lmr-PLA₂ and crotoxin B (CB), a PLA₂ from Crotalus durissus terrificus venom. Lmr-PLA₂ was isolated by molecular exclusion and reversed phase chromatography. The purified enzyme showed a molecular mass of 13,975 Da, pI of 5.46 and its partial amino acid sequence showed a high identity with PLA₂s already described in the literature. In addition, this enzyme possesses the residue D49 in its amino acid sequence, indicating that it is a catalytically active PLA₂. Lmr-PLA₂ presented high phospholipase activity and was able to inhibit platelet aggregation. Studies of biochemical characterization of new PLA₂s, as Lmr-PLA₂, are relevant since they help to clarify the structure-function relationship of this important class of toxins.

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Cordeiro A. Francielle, Perini G.K. Tibério, Bregge-Silva Cristiane, Cremonez M. Caroline, Rodrigues S. Renata, Boldrini-França Johara, C.F. Bordon de Karla, De Souza L.N. Dayane, Ache C. David, M. Rodrigues de Veridiana, dos Santos F. Wagner, Rosa C. Jose and Arantesa C. Eliane, A New Phospholipase A₂ from Lachesis muta rhombeata: Purification, Biochemical and Comparative Characterization with Crotoxin B, Protein & Peptide Letters 2015; 22 (9) . https://dx.doi.org/10.2174/0929866522666150706112431