Abstract
This review introduces the concept of enzyme biosensors. As the major feature of an enzyme is its selectivity and specificity, we briefly highlighted the principles of enzyme production and enzyme classification. Moreover, enzyme immobilization methods, including physical adsorption and entrapment techniques with special attention on cross-linking and covalent binding are discussed followed by different electrochemical methods used in the biosensors fabrication. Furthermore, a comprehensive discussion about horseradish peroxidase (HRP) as a model enzyme is presented with investigation of some modified enzyme electrodes such as screen-printed and carbon paste electrodes. Some approaches to enhance biosensors performances and their applications are also highlighted.
Keywords: Biosensors, enzyme, electrochemistry, immobilization techniques, modified electrodes.
Graphical Abstract
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