Prevention of Serum Albumin Glycation/Fibrillation by β-Cyclodextrin Functionalized Magnetic Nanoparticles

Title:Prevention of Serum Albumin Glycation/Fibrillation by β-Cyclodextrin Functionalized Magnetic Nanoparticles

Volume: 22 Issue: 7

Author(s): Mojtaba Ansari, Mehran Habibi-Rezaei*, Soheila Salahshour-Kordestani, Ali A. Moosavi Movahedi and Najmeh Poursasan

Affiliation:

• School of Biology, College of Science, University of Tehran, Tehran, Iran.,Iran

Keywords: Aggregation, β-cyclodextrin, diabetes, fibrillation, magnetic nanoparticles.

Abstract: Nowadays, glycation induced protein aggregation and related opposing strategies have received much attention. We present the effect of functionalized magnetic core–shell nanoparticles of Fe₃O₄ (MNPs) with β-cyclodextrin (β-CD) on the aggregation/fibrillation of bovine serum albumin (BSA) under diabetic condition known as amyloidogenesis. To confirm the β-CD conjugation on MNP, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM) methods were applied. Moreover, spectrofluorimetry and spectropolarimetry were utilized to analyze the effect of β-CD/Fe₃O₄ MNPs on the aggregation and amyloidogenesis of BSA through glycation. The BSA amyloidogenesis was significantly inhibited by interfering β-CD-MNPs that may present the possible diagnostic and preventive applications against the degenerative effects of protein glycation/fibrillation under diabetes.

Cite this article as:

Ansari Mojtaba, Habibi-Rezaei Mehran*, Salahshour-Kordestani Soheila, Moosavi Movahedi Ali A. and Poursasan Najmeh, Prevention of Serum Albumin Glycation/Fibrillation by β-Cyclodextrin Functionalized Magnetic Nanoparticles, Protein & Peptide Letters 2015; 22 (7) . https://dx.doi.org/10.2174/0929866522666150511150827