Stabilization of Human Serum Albumin against Urea Denaturation by Diazepam and Ketoprofen

Title:Stabilization of Human Serum Albumin against Urea Denaturation by Diazepam and Ketoprofen

Volume: 22 Issue: 7

Author(s): Pralad Manoharan, Yin H. Wong and Saad Tayyab

Affiliation:

Keywords: Diazepam, Fluorescence spectroscopy, Human serum albumin, Ketoprofen, Protein stabilization, Urea denaturation.

Abstract: Stabilizing effect of diazepam and ketoprofen, Sudlow’s site II markers on human serum albumin (HSA) against urea denaturation was studied using fluorescence spectroscopy. The two-step, three-state urea transition of HSA was transformed into a single-step, two-state transition with the abolishment of the intermediate state along with a shift of the transition curve towards higher urea concentrations in the presence of diazepam or ketoprofen. Interestingly, a greater shift in the transition curve of HSA was observed in the presence of ketoprofen compared to diazepam. A comparison of the intrinsic fluorescence and three-dimensional fluorescence spectra of HSA and partially-denatured HSAs, obtained in the absence and the presence of diazepam or ketoprofen suggested significant retention of native-like conformation in the partially-denatured states of HSA in the presence of Sudlow’s site II markers. Taken together, all these results suggested stabilization of HSA in the presence of diazepam or ketoprofen, being greater in the presence of ketoprofen.

Cite this article as:

Manoharan Pralad, Wong H. Yin and Tayyab Saad, Stabilization of Human Serum Albumin against Urea Denaturation by Diazepam and Ketoprofen, Protein & Peptide Letters 2015; 22 (7) . https://dx.doi.org/10.2174/0929866522666150506141714

DOI https://dx.doi.org/10.2174/0929866522666150506141714	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305