白芍内酯苷、芦荟泻素和新橙皮苷对β-淀粉样蛋白聚合物的抑制作用及其减低β-淀粉样蛋白导致的毒性以发挥对主要海马细胞的神经保护作用

Title:Inhibition of β-Amyloid Aggregation by Albiflorin, Aloeemodin and Neohesperidin and their Neuroprotective Effect on Primary Hippocampal Cells Against β-Amyloid Induced Toxicity

Volume: 12 Issue: 5

Author(s): See-Lok Ho, Chung-Yan Poon, Chengyuan Lin, Ting Yan, Daniel Wai-Jing Kwong and Ken Kin-Lam Yung, Man S. Wong, Zhaoxiang Bian and Hung-Wing Li

Affiliation:

关键词: 淀粉样蛋白聚合物；钙流入；草本植物；抑制剂；神经保护作用；活性氧

摘要: Being one of the hallmarks of Alzheimer’s disease, β-amyloid (Aβ) aggregates induce complicated neurotoxicity. Evidences show that the underlying mechanism of neurotoxicity involves a glutamate receptor subtype, N-methyl-D-aspartate (NMDA) receptor, an increase in intracellular calcium( II) ion loading as well as an elevation in oxidation stress. In this work, among the 35 chemical components of Chinese herbal medicines (CHMs) being screened for inhibitors of Aβ aggregation, four of them, namely albiflorin, aloeemodin, neohesperidin and physcion, were found for the first time to exhibit a potent inhibitory effect on Aβ_1-40 and Aβ_1-42 aggregation. Their neuroprotective capability on primary hippocampal neuronal cells was also investigated by MTT assay, ROS assay and intracellular calcium(II) ion concentration measurement. It was interesting to find that physcion was rather toxic to neuronal cells while albiflorin, aloeemodin and neohesperidin reduced the toxicity and ROS induced by both monomeric and oligomeric Aβ species. In addition, albiflorin was particularly powerful in maintaining the intracellular Ca²⁺ concentration.

Cite this article as:

See-Lok Ho, Chung-Yan Poon, Chengyuan Lin, Ting Yan, Daniel Wai-Jing Kwong and Ken Kin-Lam Yung, Man S. Wong, Zhaoxiang Bian and Hung-Wing Li , Inhibition of β-Amyloid Aggregation by Albiflorin, Aloeemodin and Neohesperidin and their Neuroprotective Effect on Primary Hippocampal Cells Against β-Amyloid Induced Toxicity, Current Alzheimer Research 2015; 12 (5) . https://dx.doi.org/10.2174/1567205012666150504144919