Abstract
The present study was undertaken to investigate the preliminary phytochemical screening and antispermatogenic activity of different extracts of Artocarpus lakoocha Roxb fruits and the major constituent isolated from the active extract. The powdered plant material was defatted with petroleum ether and then successively extracted with chloroform, ethanol and distilled water. The prepared extracts were screened for the presence of various phytoconstituents, which showed the presence of alkaloids, steroids, carbohydrates, glycosides, saponin, protein and phenolic compounds. Petroleum ether, chloroform, ethanolic and aqueous extracts of Artocarpus lakoocha fruits were tested for their antispermatogenic potential, at the doses of 100, 200 and 300mg/kg, body weight, p.o., for 45 days. The chloroform extract of fruit (at a dose of 300 mg/kg body weight) was found to be most active among all. Therefore, it was subjected to the isolation of phytoconstituent responsible for the activity using column chromatography. The isolated compound was characterized by TLC, melting point, UV, IR, 1H-NMR and Mass spectroscopy and was identified as β-sitosterol. Antispermatogenic activity of the isolated compound was evaluated at a dose of 5 mg/kg body weight/day, for 45 days. The treatment caused significant decrease (P<0.01) in the weight of reproductive organs such as; testis, epididymis and seminal vesicle. Moreover, the sperm count, sperm viability and serum testosterone levels were significantly lowered when compared to that of the control group.
Keywords: Anti-spermatogenic activity, Artocarpus lakoocha, natural contraceptive, β-Sitosterol, testosterone.
Graphical Abstract
The Natural Products Journal
Title:Artocarpus lakoocha Fruits: Phytochemical Investigation and Anti- Spermatogenic Potential
Volume: 5 Issue: 1
Author(s): Bidhyut K. Dubey, Kiran Singh, Avinash C. Tripathi, Mary Koshy and Shailendra K. Saraf
Affiliation:
Keywords: Anti-spermatogenic activity, Artocarpus lakoocha, natural contraceptive, β-Sitosterol, testosterone.
Abstract: The present study was undertaken to investigate the preliminary phytochemical screening and antispermatogenic activity of different extracts of Artocarpus lakoocha Roxb fruits and the major constituent isolated from the active extract. The powdered plant material was defatted with petroleum ether and then successively extracted with chloroform, ethanol and distilled water. The prepared extracts were screened for the presence of various phytoconstituents, which showed the presence of alkaloids, steroids, carbohydrates, glycosides, saponin, protein and phenolic compounds. Petroleum ether, chloroform, ethanolic and aqueous extracts of Artocarpus lakoocha fruits were tested for their antispermatogenic potential, at the doses of 100, 200 and 300mg/kg, body weight, p.o., for 45 days. The chloroform extract of fruit (at a dose of 300 mg/kg body weight) was found to be most active among all. Therefore, it was subjected to the isolation of phytoconstituent responsible for the activity using column chromatography. The isolated compound was characterized by TLC, melting point, UV, IR, 1H-NMR and Mass spectroscopy and was identified as β-sitosterol. Antispermatogenic activity of the isolated compound was evaluated at a dose of 5 mg/kg body weight/day, for 45 days. The treatment caused significant decrease (P<0.01) in the weight of reproductive organs such as; testis, epididymis and seminal vesicle. Moreover, the sperm count, sperm viability and serum testosterone levels were significantly lowered when compared to that of the control group.
Export Options
About this article
Cite this article as:
K. Dubey Bidhyut, Singh Kiran, C. Tripathi Avinash, Koshy Mary and K. Saraf Shailendra, Artocarpus lakoocha Fruits: Phytochemical Investigation and Anti- Spermatogenic Potential, The Natural Products Journal 2015; 5 (1) . https://dx.doi.org/10.2174/2210315505666150219233415
DOI https://dx.doi.org/10.2174/2210315505666150219233415 |
Print ISSN 2210-3155 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-3163 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers