线粒体氧化呼吸相关的基因分析揭示了细胞核和线粒体基因在异源四倍体杂交的联合作用

Title:Analysis of Mitochondrial Respiratory-Related Genes Reveals Nuclear and Mitochondrial Genome Cooperation in Allotetraploid Hybrid

Volume: 14 Issue: 10

Author(s): L.-Y. Peng, J. Wang, M. Tao, C.-P. You, L. Ye, J. Xiao, C. Zhang, Y. Liu and S.-J. Liu

Affiliation:

关键词: 异源四倍体，协作，基因重复，线粒体DNA，细胞核基因

摘要: An allotetraploid hybrid lineage derived from the distant hybridization of red crucian carp (Carassius auratus red var., ♀, 2n =100) × common carp (Cyprinus carpio L., ♂, 2n =100) was investigated for its mitochondrial and nuclear genome inheritance patterns. Based on liver transcriptomic data for this hybrid, red crucian carp, and common carp, we identified 94, 136, and 86 contigs corresponding to 41, 46, and 37 mitochondrial respiratory chain nuclear genes, respectively. Mitochondrial respiratory chain nuclear gene sequences from red crucian carp and common carp were both detected in the allotetraploid hybrid, indicating that both parental nuclear genomes were participated in the synthesis of mitochondrial respiratory protein complexes in the hybrid. For mitochondrial respiratory related genes, high sequence similarity (>90%) and a low nucleotide divergence rate (<0.2) between red crucian carp and common carp could be a critical factor allowing cooperation of the three genomes (red crucian carp mitochondrial genome, red crucian and common carp nuclear genomes) in the allotetraploid hybrid lineage. Interestingly, gene duplication events were identified in the allotetraploid hybrid, red crucian and common carp, as confirmed by analysis of orthologous gene trees for these fish. Our findings provide valuable information with which to study cooperation between the nuclear and mitochondrial genomes of other hybrids, and will provide basic genetic information of relevance to mitochondrial-related diseases in humans and animals.

Cite this article as:

L.-Y. Peng, J. Wang, M. Tao, C.-P. You, L. Ye, J. Xiao, C. Zhang, Y. Liu and S.-J. Liu , Analysis of Mitochondrial Respiratory-Related Genes Reveals Nuclear and Mitochondrial Genome Cooperation in Allotetraploid Hybrid, Current Molecular Medicine 2014; 14 (10) . https://dx.doi.org/10.2174/1566524014666141203095503