Characterization and Evaluation of Cellulases and Hemicellulases Produced by Diverse Fungal Strains

Title:Characterization and Evaluation of Cellulases and Hemicellulases Produced by Diverse Fungal Strains

Volume: 3 Issue: 3

Author(s): Amandeep Monga and B.S. Chadha

Affiliation:

Keywords: Enzyme activities, ethanol production, glycosyl hydrolases, PAGE, secretome.

Abstract: Sixteen mesophilic fungal strains of Aspergilli (10) and Penicilli (3) and Cochliobolus lunatus isolated from deciduous green forests and wetlands were characterized at molecular level and screened for cellulases (endoglucanase, β- glucosidase (cellobiase), cellobiohydrolase (CBHI/EGI) and FPase) and hemicellulase (xylanase, α -Larabinofuranosidase, β -xylosidase, acetyl xylan esterase and feruloyl esterase) production on rice straw & wheat bran containing solidified substrate. Penicillium janthinellum (3CHP) was found to produce high levels of β-glucosidase and α- L-arabinofuranosidase activities, whereas, Aspergillus tubingensis (8 DAL) produced maximal levels of endoglucanase, Fpase, xylanase and β-xylosidase. The cultures extracts from different strains were analysed for expression of multiple isoforms of endoglucanases, β-glucosidases, xylanases, α-L-arabinofuranosidases, β-xylosidases and acetyl xylan esterase. The enzyme cocktails were used for saccharification of alkali treated rice straw at substrate loading rate of (7%). The maximal saccharification (56%) was observed in enzymatic hydrolysates from Aspergillus terreus.

Cite this article as:

Monga Amandeep and Chadha B.S., Characterization and Evaluation of Cellulases and Hemicellulases Produced by Diverse Fungal Strains, Current Biotechnology 2014; 3 (3) . https://dx.doi.org/10.2174/2211550103666140820225131