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Current Stem Cell Research & Therapy

Editor-in-Chief

ISSN (Print): 1574-888X
ISSN (Online): 2212-3946

VEGF, BMP-7, MatrigelTM, Hyaluronic Acid, In Vitro Cultured Chondrocytes and Trephination for Healing of the Avascular Portion of the Meniscus. An Experimental Study in Sheep

Author(s): Francisco Forriol, Umile Giuseppe Longo, Julio Duart, Purification Ripalda, Javier Vaquero, Mattia Loppini, Giovanni Romeo, Stefano Campi, Wasim S. Khan, Andrea O. Muda and Vincenzo Denaro

Volume 10, Issue 1, 2015

Page: [69 - 76] Pages: 8

DOI: 10.2174/1574888X09666140710115356

Price: $65

Abstract

Objective: To evaluate the effects of VEGF, BMP-7, MatrigelTM, hyaluronic acid, in vitro cultured chondrocytes and trephination to promote and enhance the healing process of avascular meniscal tears in an animal model. Methods: A longitudinal tear was produced in the inner avascular part of the meniscus of 24 sheeps. Each tear was treated with trephination technique and suture. The animals were divided into 6 groups to receive a different treatment: control (I); VEGF, BMP-7, MatrigelTM, hyaluronic acid, in vitro cultured chondrocytes. At 8 weeks from surgery, meniscal samples were explanted and analyzed by histology, immunohistochemistry, and histomorphometry. Results: At the histological examination, Group IV and VI showed a partial closure of the meniscal lesion, whereas Group I, II, III, and V did not show any evidence of healing. In the group IV, the healed tissue represented the 22.95% of the lesion area. In the group VI, the healed tissue represented the 43.75% of the lesion area. Conclusions: Autologous chondrocytes and BMP-7 associated with trephination and suture techniques enhanced healing process of meniscal tears in the avascular inner third of the meniscus in ovine model.

Keywords: BMP-7, cell-therapy, healing, growth factors, meniscus, repair.


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