抗乙肝病毒感染的治疗性DNA疫苗研究进展及发展中所面临的挑战

Title:Advances and Challenges in the Development of Therapeutic DNA Vaccines Against Hepatitis B Virus Infection

Volume: 14 Issue: 3

Author(s): Lucyna Cova

Affiliation:

关键词: DNA疫苗，乙肝病毒，土拨鼠乙肝病毒（WHB），鸭乙肝病毒（DHBV），乙型肝炎，电穿孔法，免疫疗法

摘要: Despite the existence of an effective prophylactic vaccine, chronic hepatitis B virus (HBV) infection remains a major public health problem. Because very weak and functionally impaired virus-specific immune responses play a key role in the persistence of HBV infection, the stimulation of these responses appears to be of particular importance for virus clearance. In this regard DNA-based vaccination has emerged as novel, promising therapeutic approach for chronic hepatitis B. This review provides an update of preclinical studies in animal models (mouse, chimpanzee, duck, woodchuck), which evaluated the ability of DNA vaccines targeting hepadnaviral proteins to induce potent and sustained immune responses in naïve animals and to enhance virus clearance and break immune tolerance in chronic virus-carriers. Different strategies have been developed and evaluated in these models to optimize DNA vaccine including genetic adjuvants, combination with antiviral drugs, prime-boost regimens and plasmid delivery. The delivery of DNA by in vivo electroporation appears to be of particular interest for increase of vaccine potency in both small and large animal models. Based on the promising results generated in preclinical studies, first clinical trials of DNA vaccines have been initiated, although effective therapy of chronic hepatitis B awaits further improvements in vaccine efficacy.

Cite this article as:

Cova Lucyna, Advances and Challenges in the Development of Therapeutic DNA Vaccines Against Hepatitis B Virus Infection, Current Gene Therapy 2014; 14 (3) . https://dx.doi.org/10.2174/1566523214666140509102644