Different Approaches for Obtaining Antibodies from Human B Cells

Title:Different Approaches for Obtaining Antibodies from Human B Cells

Volume: 11 Issue: 1

Author(s): Marcus R. Duvall and Ryan N. Fiorini

Affiliation:

Keywords: Antibody engineering, antibody libraries, display technologies, high-throughput screening, human monoclonal antibodies, human B cell.

Abstract: Antibodies have emerged as powerful therapeutics effective for treating a number of human conditions and diseases. While early successes utilized small animals to generate therapeutic antibodies, human antibodies are now preferred in order to limit anti-antibody immune responses. Antibodies with human amino acid sequences can be generated in a number of ways, such as humanizing antibodies from other species or expressing human antibodies in transgenic animals. This review focuses on methods for obtaining antibodies directly from human B cells. These methods use both antigen exposed and non-exposed (“naïve”) humans as B cell sources, and apply various technologies to isolate desired antibodies; including cell line generation, single cell isolation, display technologies, and B cell library generation.

Cite this article as:

Duvall R. Marcus and Fiorini N. Ryan, Different Approaches for Obtaining Antibodies from Human B Cells, Current Drug Discovery Technologies 2014; 11 (1) . https://dx.doi.org/10.2174/1570163811666140121154649