Covalent Immobilization of α-Glucosidase Enzyme onto Amine Functionalized Multi-walled Carbon Nanotubes

Title:Covalent Immobilization of α-Glucosidase Enzyme onto Amine Functionalized Multi-walled Carbon Nanotubes

Volume: 10 Issue: 5

Author(s): M. Mohiuddin, D. Arbain, A.K.M. Shafiqul Islam, M. Rahman, M.S. Ahmad and M.N. Ahmad

Affiliation:

Keywords: Cyclic voltammetry, functionalization, α-glucosidase, immobilization.

Abstract: α-glucosidase (AG) enzyme was immobilized onto amine functionalized multi-walled carbon nanotubes (MWCNTs-NH₂) by treatment with cross linkers, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) via amide bonding. The immobilized AG was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). The performance of the electrode was evaluated by Spectrophotometric method and cyclic voltammetric (CV) method using p-nitrophenyl-α-D-glucopyranoside (PNPG) as a substrate. The immobilized AG enzyme retains about 84.16% activity of its initial response even after 30 days when stored at 4^°C.

Cite this article as:

Mohiuddin M., Arbain D., Islam Shafiqul A.K.M., Rahman M., Ahmad M.S. and Ahmad M.N., Covalent Immobilization of α-Glucosidase Enzyme onto Amine Functionalized Multi-walled Carbon Nanotubes, Current Nanoscience 2014; 10 (5) . https://dx.doi.org/10.2174/1573413710666140116213526