Protective Effect of α-ketobutyrate on Survival of Hippocampal Neurons Challenged with Hydrogen Peroxide Chemistry Mimicking Brain Ischemia

Title:Protective Effect of α-ketobutyrate on Survival of Hippocampal Neurons Challenged with Hydrogen Peroxide Chemistry Mimicking Brain Ischemia

Volume: 10 Issue: 2

Author(s): Dagmara Jacewicz, Kamila Siedlecka-Kroplewska, Joanna Pranczk, Urszula Popowska, Alicja Kuban-Jankowska, Narcyz Knap, Dariusz Wyrzykowski, Krzysztof Zamojc, Michal Wozniak and Lech Chmurzyrski

Affiliation:

Keywords: Neuronal cells, molecular biosensor of CO₂, chromium(III) complex.

Abstract: A protective effect of α -ketobutyrate on hippocampal neuronal cells treated with H₂O₂ was tested. α - Ketobutyrate concentrations were as follows: 0.5 mM, 1 mM and 5 mM. Tests were performed at the temperature of 37 ºC. Moreover, the protective effect of α-ketobutyrate on the cytotoxicity of 100 and 40 nmol/4·10³ cells treated by hydrogen peroxide was observed. Furthermore, the role of α-ketobutyrate as an energy substrate was also proven using the cis- [Cr(C₂O₄)(pm)(OH₂)₂]⁺ complex as a biosensor for the carbon dioxide uptake process. All the experiments were carried out under in-vitro conditions.

Cite this article as:

Jacewicz Dagmara, Siedlecka-Kroplewska Kamila, Pranczk Joanna, Popowska Urszula, Kuban-Jankowska Alicja, Knap Narcyz, Wyrzykowski Dariusz, Zamojc Krzysztof, Wozniak Michal and Chmurzyrski Lech, Protective Effect of α-ketobutyrate on Survival of Hippocampal Neurons Challenged with Hydrogen Peroxide Chemistry Mimicking Brain Ischemia, Current Pharmaceutical Analysis 2014; 10 (2) . https://dx.doi.org/10.2174/1573412909666131231231631