Abstract
With this study we intend to evaluate the effects of 8 different glucosinolate hydrolysis products including isothiocyanates as antibacterial compounds against Aeromonadaceae, isolated from intestinal segments of pigs collected directly from slaughter-houses in the North of Portugal. Four Aeromonas species, A. allosaccharophila, A. hydrophila, A. media and A. veronii were identified. Using disc-diffusion bioassay all compounds were tested at 6 different doses (0.015, 0.15, 0.75, 1.5 and 3.0 µmoles) in dimethyl-sulfoxide (DMSO). Antibiotic standard (gentamicin) and DMSO (negative control) were included in all experiments. Minimum and maximum dose inhibitions (in µmoles) were assessed. To our knowledge, this is the first study of antimicrobial activity of glucosinolate hydrolysis products against Aeromonas species. The results showed that glucosinolate hydrolysis products and particularly the isothiocyanates have antimicrobial activity, which was proportional to the concentration used. However, not all revealed the same tendency, which means that the chemical structure of each compound is fundamental to understand their effectiveness. Among the different isothiocyanates the benzylisothiocyanate, sulforaphane and 2-phenylethylisothiocyanate were the most effective in vitro inhibitors of bacterial growth. This in vitro study provides enough data to demonstrate the potential use of these natural dietary chemicals for treating infectious diseases caused by Aeromonas spp.
Keywords: Aeromonadaceae, antibacterial activity, drug screening, isothiocyanates, natural products.
Medicinal Chemistry
Title:Antimicrobial Susceptibility of Aeromonas Spp. Isolated from Pig Ileum Segments to Natural Isothiocyanates
Volume: 9 Issue: 6
Author(s): Alfredo Aires, Carla S.P. Dias, Eduardo A. S. Rosa and Maria Jose Saavedra
Affiliation:
Keywords: Aeromonadaceae, antibacterial activity, drug screening, isothiocyanates, natural products.
Abstract: With this study we intend to evaluate the effects of 8 different glucosinolate hydrolysis products including isothiocyanates as antibacterial compounds against Aeromonadaceae, isolated from intestinal segments of pigs collected directly from slaughter-houses in the North of Portugal. Four Aeromonas species, A. allosaccharophila, A. hydrophila, A. media and A. veronii were identified. Using disc-diffusion bioassay all compounds were tested at 6 different doses (0.015, 0.15, 0.75, 1.5 and 3.0 µmoles) in dimethyl-sulfoxide (DMSO). Antibiotic standard (gentamicin) and DMSO (negative control) were included in all experiments. Minimum and maximum dose inhibitions (in µmoles) were assessed. To our knowledge, this is the first study of antimicrobial activity of glucosinolate hydrolysis products against Aeromonas species. The results showed that glucosinolate hydrolysis products and particularly the isothiocyanates have antimicrobial activity, which was proportional to the concentration used. However, not all revealed the same tendency, which means that the chemical structure of each compound is fundamental to understand their effectiveness. Among the different isothiocyanates the benzylisothiocyanate, sulforaphane and 2-phenylethylisothiocyanate were the most effective in vitro inhibitors of bacterial growth. This in vitro study provides enough data to demonstrate the potential use of these natural dietary chemicals for treating infectious diseases caused by Aeromonas spp.
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Cite this article as:
Aires Alfredo, Dias S.P. Carla, Rosa A. S. Eduardo and Saavedra Jose Maria, Antimicrobial Susceptibility of Aeromonas Spp. Isolated from Pig Ileum Segments to Natural Isothiocyanates, Medicinal Chemistry 2013; 9 (6) . https://dx.doi.org/10.2174/1573406411309060010
DOI https://dx.doi.org/10.2174/1573406411309060010 |
Print ISSN 1573-4064 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6638 |
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