Abstract
The generation of genetically altered mice for research is invaluable for investigations into the underlying mechanisms of common complex diseases such as cancer as well as for therapeutic studies. The correct identification of the genotype of each mouse is therefore essential. Genotyping is routinely performed by using blood, tail or ear tissue; however, this can cause pain or discomfort to the animal and nor is amenable to scale up for high-throughput sampling and analyses. Genotyping from saliva, hair and rectal cells is less invasive, but still requires the restraint of the animal. In contrast, collection of stool samples for DNA isolation is non-invasive as well as repeatable and time efficient, with recent studies showing it to be as accurate as the alternative methods. Fecal DNA genotyping therefore provides an attractive technique for non-invasive genotyping. This feature article examines and synthesizes the recent advances in fecal genotyping with a view to mouse models for nutrigenomics cancer chemoprevention studies specifically, and personalized medicine more broadly.
Keywords: Cancer, feces, genotyping, non-invasive, novel diagnostic platforms, personalized medicine, transgenic mice
Current Pharmacogenomics and Personalized Medicine
Title:Fecal DNA Genotyping: A Non-invasive Approach to Characterize Mouse Models for Nutrigenomics Cancer Chemoprevention Studies
Volume: 11 Issue: 1
Author(s): Erin L. Symonds
Affiliation:
Keywords: Cancer, feces, genotyping, non-invasive, novel diagnostic platforms, personalized medicine, transgenic mice
Abstract: The generation of genetically altered mice for research is invaluable for investigations into the underlying mechanisms of common complex diseases such as cancer as well as for therapeutic studies. The correct identification of the genotype of each mouse is therefore essential. Genotyping is routinely performed by using blood, tail or ear tissue; however, this can cause pain or discomfort to the animal and nor is amenable to scale up for high-throughput sampling and analyses. Genotyping from saliva, hair and rectal cells is less invasive, but still requires the restraint of the animal. In contrast, collection of stool samples for DNA isolation is non-invasive as well as repeatable and time efficient, with recent studies showing it to be as accurate as the alternative methods. Fecal DNA genotyping therefore provides an attractive technique for non-invasive genotyping. This feature article examines and synthesizes the recent advances in fecal genotyping with a view to mouse models for nutrigenomics cancer chemoprevention studies specifically, and personalized medicine more broadly.
Export Options
About this article
Cite this article as:
L. Symonds Erin, Fecal DNA Genotyping: A Non-invasive Approach to Characterize Mouse Models for Nutrigenomics Cancer Chemoprevention Studies, Current Pharmacogenomics and Personalized Medicine 2013; 11 (1) . https://dx.doi.org/10.2174/1875692111311010004
DOI https://dx.doi.org/10.2174/1875692111311010004 |
Print ISSN 1875-6921 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6913 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Nanofibers Based Tissue Engineering and Drug Delivery Approaches for Myocardial Regeneration
Current Pharmaceutical Design Noninvasive Monitoring of Intracranial Pressure
Recent Patents on Biomedical Engineering (Discontinued) In Vitro and In Vivo Neutralizing Activity of Uvaria chamae Leaves Fractions on the Venom of Naja nigricollis in Albino Rat and Bovine Blood
Recent Patents on Biotechnology Abnormal Saccadic Intrusions with Alzheimer's Disease in Darkness
Current Alzheimer Research Neurotransmitter Effects in Human Immunodeficiency Virus (HIV) and Simian Immuno-Deficiency Virus (SIV) Infection
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Beyond Mitochondria, What Would be the Energy Source of the Cell?
Central Nervous System Agents in Medicinal Chemistry Novel Penetrating Cations for Targeting Mitochondria
Current Pharmaceutical Design The Mitochondrial Dynamics of Alzheimers Disease and Parkinsons Disease Offer Important Opportunities for Therapeutic Intervention
Current Pharmaceutical Design Adiposity and the Gut - The Role of Gut Hormones
Current Nutrition & Food Science Molecular Dynamics Simulations of Intrinsically Disordered Proteins in Human Diseases
Current Computer-Aided Drug Design Sleep-Disordered Breathing and Cardiovascular Disease: Exploring Pathophysiology and Existing Data
Current Respiratory Medicine Reviews Small Animal Computed Tomography Imaging
Current Medical Imaging Potential Effects of Cannabidiol as a Wake-Promoting Agent
Current Neuropharmacology Application of Vibrational Spectroscopy to Study Solid-state Transformations of Pharmaceuticals
Current Pharmaceutical Design Clostridial Neurotoxins: Mode of Substrate Recognition and Novel Therapy Development
Current Protein & Peptide Science Spinal Muscular Atrophy: Molecular Mechanisms
Current Molecular Medicine Docking Screens: Right for the Right Reasons?
Current Topics in Medicinal Chemistry Activity-dependent Synaptic Plasticity and Metaplasticity in Spinal Motor Networks
Current Pharmaceutical Design Proceedings of the Regional Conference of the International Society for Adolescent Psychiatry and Psychology (ISAPP), a Joint Conference with 18<sup>th</sup> National Symposium of Adolescent Mental Health, Ankara, Turkey, November 21-24, 2013<sup>1</sup>
Adolescent Psychiatry Tuberculosis, an Old Disease Lacking New Therapeutic Drugs
Current Drug Therapy