Abstract
Pseudomonas aeruginosa MTCC 7925, a sludge isolate, was found to synthesize a novel short-chain-lengthlong- chain-length (SCL-LCL) co-polymer with 3-hydroxybutyric acid (3HB), 3-hydroxyvaleric acid (3HV), 3- hydroxyhexadecanoic acid (3HHD) and 3-hydroxyoctadecanoic acid (3HOD) as constituents. Under batch mode study, cells harvested at the stationary phase of growth depicted maximum PHAs accumulation, i.e. 24% of dry cell weight (dcw) at 48 h of incubation. The co-polymer accumulation was raised to 49% (dcw) under 2% ethanol-supplemented condition. A further, rise up to 78% (dcw) was recorded by optimizing the critical variables by Response Surface Methodology (RSM). When palm oil and its cakes were used as carbon sources, yield of 5.9 g/l was achieved, which was almost 85-fold higher against control. The thermal and mechanical properties of the polymer are comparable with polypropylene and polyethylene, thus opening new possibilities for various industrial applications.
Keywords: Inexpensive carbon sources, N-deficiency, P-deficiency, PHAs, Pseudomonas aeruginosa MTCC 7925, RSM, SCL-LCL-PHA co-polymer
Current Biotechnology
Title:Pseudomonas aeruginosa MTCC 7925: Producer of a Novel SCL-LCLPHA Co-Polymer
Volume: 2 Issue: 1
Author(s): Akhilesh Kumar Singh, Ranjana Bhati, Shilalipi Samantaray and Nirupama Mallick
Affiliation:
Keywords: Inexpensive carbon sources, N-deficiency, P-deficiency, PHAs, Pseudomonas aeruginosa MTCC 7925, RSM, SCL-LCL-PHA co-polymer
Abstract: Pseudomonas aeruginosa MTCC 7925, a sludge isolate, was found to synthesize a novel short-chain-lengthlong- chain-length (SCL-LCL) co-polymer with 3-hydroxybutyric acid (3HB), 3-hydroxyvaleric acid (3HV), 3- hydroxyhexadecanoic acid (3HHD) and 3-hydroxyoctadecanoic acid (3HOD) as constituents. Under batch mode study, cells harvested at the stationary phase of growth depicted maximum PHAs accumulation, i.e. 24% of dry cell weight (dcw) at 48 h of incubation. The co-polymer accumulation was raised to 49% (dcw) under 2% ethanol-supplemented condition. A further, rise up to 78% (dcw) was recorded by optimizing the critical variables by Response Surface Methodology (RSM). When palm oil and its cakes were used as carbon sources, yield of 5.9 g/l was achieved, which was almost 85-fold higher against control. The thermal and mechanical properties of the polymer are comparable with polypropylene and polyethylene, thus opening new possibilities for various industrial applications.
Export Options
About this article
Cite this article as:
Kumar Singh Akhilesh, Bhati Ranjana, Samantaray Shilalipi and Mallick Nirupama, Pseudomonas aeruginosa MTCC 7925: Producer of a Novel SCL-LCLPHA Co-Polymer, Current Biotechnology 2013; 2 (1) . https://dx.doi.org/10.2174/2211550111302010013
DOI https://dx.doi.org/10.2174/2211550111302010013 |
Print ISSN 2211-5501 |
Publisher Name Bentham Science Publisher |
Online ISSN 2211-551X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
MicroRNA (miR)-429 Promotes Inflammatory Injury by Targeting Kruppel-like Factor 4 (KLF4) in Neonatal Pneumonia
Current Neurovascular Research Pain and Child: A Translational Hypothesis on the Pathophysiology of a Mild Type-2 Diabetes Model
Endocrine, Metabolic & Immune Disorders - Drug Targets Congenital Heart Disease: The Crossroads of Genetics, Epigenetics and Environment
Current Genomics Actigraphy for Assessing Light Effects on Sleep and Circadian Activity Rhythm in Alzheimer's Dementia: A Narrative Review
Current Alzheimer Research Does Mechanism of Drug Action Matter to Inform Rational Polytherapy in Epilepsy?
CNS & Neurological Disorders - Drug Targets The Potential Therapeutic Role of the HMGB1-TLR Pathway in Epilepsy
Current Drug Targets Indole Alkaloids and Semisynthetic Indole Derivatives as Multifunctional Scaffolds Aiming the Inhibition of Enzymes Related to Neurodegenerative Diseases – A Focus on Psychotria L. Genus
Current Topics in Medicinal Chemistry Calcium Signaling in Mammalian Eggs at Fertilization
Current Topics in Medicinal Chemistry Diet and Neurocognition: Review of Evidence and Methodological Considerations
Current Aging Science Mechanisms in the Bed Nucleus of the Stria Terminalis Involved in Control of Autonomic and Neuroendocrine Functions: A Review
Current Neuropharmacology Recent Developments in Deception Research
Current Psychiatry Reviews Blood-brain Barrier Disruption Leads to Postoperative Cognitive Dysfunction
Current Neurovascular Research Drug Delivery Systems for Imaging and Therapy of Parkinson's Disease
Current Neuropharmacology Medical Treatment of Malignancy-Associated Hypercalcemia
Current Medicinal Chemistry Is there Any Correlation Between Binding and Functional Effects at the Translocator Protein (TSPO) (18 kDa)?
Current Molecular Medicine Graphene Modified Molecular Imprinting Electrochemical Sensor for Determining the Content of Dopamine
Current Analytical Chemistry Neurochemical Markers in the Mammalian Brain: Structure, Roles in Synaptic Communication, and Pharmacological Relevance
Current Medicinal Chemistry Involvement of Mast Cells in Angiogenesis and Chronic Inflammation
Current Drug Targets - Inflammation & Allergy Editorial [Hot Topic: Recent Advances and Future Prospect in Protease Targeting (Executive Editor: B. Turk)]
Current Pharmaceutical Design Obesity, Diabetes and Cardiovascular Diseases in India: Public Health Challenges
Current Diabetes Reviews