Abstract
HIF-1α is regarded as a target for drug development in several diseases such as cancer. For high throughput screening of HIF-1α-targeted drug, we need to examine the activity quantitatively. In the present study, we proposed a method where stable expression system of HIF-1α was combined with image correlation analysis. When the stable transformants were labeled with DRAQ5, we could detect Co2+-induced nuclear translocation by the use of cross-correlation analysis of the dual labeling images. In the case of high throughput screening for HIF-1α-targeted drug, we should use Pearson’s correlation coefficient to judge nuclear translocation.
Keywords: HIF-1α, GFP, stable transformant, colocalization, cobalt, drug development, cancer, DRAQ5, dual labeling images, nuclear translocation, hypoxia response element (HRE), vessel, glycolytic enzymes, hypoxic
Current Pharmaceutical Biotechnology
Title:Image Analysis of Colocalization of Nuclear DNA and GFP Labelled HIF-1α in Stable Transformants
Volume: 13 Issue: 14
Author(s): Takehito Goto, Michihiko Sato, Eiji Takahashi and Yasutomo Nomura
Affiliation:
Keywords: HIF-1α, GFP, stable transformant, colocalization, cobalt, drug development, cancer, DRAQ5, dual labeling images, nuclear translocation, hypoxia response element (HRE), vessel, glycolytic enzymes, hypoxic
Abstract: HIF-1α is regarded as a target for drug development in several diseases such as cancer. For high throughput screening of HIF-1α-targeted drug, we need to examine the activity quantitatively. In the present study, we proposed a method where stable expression system of HIF-1α was combined with image correlation analysis. When the stable transformants were labeled with DRAQ5, we could detect Co2+-induced nuclear translocation by the use of cross-correlation analysis of the dual labeling images. In the case of high throughput screening for HIF-1α-targeted drug, we should use Pearson’s correlation coefficient to judge nuclear translocation.
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Cite this article as:
Goto Takehito, Sato Michihiko, Takahashi Eiji and Nomura Yasutomo, Image Analysis of Colocalization of Nuclear DNA and GFP Labelled HIF-1α in Stable Transformants, Current Pharmaceutical Biotechnology 2012; 13 (14) . https://dx.doi.org/10.2174/138920101314151120122519
DOI https://dx.doi.org/10.2174/138920101314151120122519 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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