Abstract
In order to investigate the enantioseparation property of the biselector chiral stationary phase derived from polysaccharide, a new biselector chiral stationary phase was prepared by coating a blend of two amylose derivatives on an aminated silica gel. The blend consisted of amylose tris(phenylcarbamate) and amylose tris(benzoate) at a glucose unit ratio of 1:1 (mol/mol). For the sake of enantioseparation comparison, the corresponding single selector chiral stationary phases were also prepared with the two individual amylose derivatives. The enantioseparation ability of these chiral stationary phases was evaluated by using structurally various chiral analytes. The biselector chiral stationary phase showed an overall improved enantioseparation ability in comparison with the two single selector chiral stationary phases. The effect of alcohols in mobile phase on the enantioseparation of the biselector chiral stationary phase was also discussed.
Keywords: Amylose, Biselector, Chiral stationary phase, Enantioseparation, High-performance liquid chromatography, Single selector
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