Abstract
The interaction between human serum albumin and cholesterol-modified pullulan (CHP) nanoparticles with different degrees of substitution (DS) of cholesterol moiety was investigated using spectroscopic and thermodynamic methods. Albumin fluorescence intensity was quenched by nanoparticles with maximum emission intensity decreasing at the initial reaction and increasing at the last reacted period. Binding constants (Kb) were 1.12 x 105 M-1, 4.12 x 105 M-1 and 7.44 x 105 M-1 to CHP-3.11, CHP-6.03 and CHP-6.91, respectively, as determined by Stern-Volmer analysis. Adsorption of albumin to nanoparticles was an exothermic reaction process and revealed a higher DS of cholesterol moiety with higher enthalpy and entropy changes. Upon interaction with nanoparticles, albumin conformation changed with a reduction of α-helix, suggesting a partial protein unfolding. Furthermore, albumin could gradually change its helical structure due to the structural change of the complexed nanoparticle. Particle hydrophobicity and shell-core structure play a main role in the alteration of albumin conformation in the nanoparticle-protein interaction process.
Keywords: Binding constant, helical structure, hydrophobicity, fluorescence intensity, spectroscopic, thermodynamic
Current Nanoscience
Title:The Interaction between Human Serum Albumin and Cholesterol-modified Pullulan Nanoparticle
Volume: 8 Issue: 6
Author(s): Xiaojun Tao, Qiufang Zhang, Wenzhi Yang and Qiqing Zhang
Affiliation:
Keywords: Binding constant, helical structure, hydrophobicity, fluorescence intensity, spectroscopic, thermodynamic
Abstract: The interaction between human serum albumin and cholesterol-modified pullulan (CHP) nanoparticles with different degrees of substitution (DS) of cholesterol moiety was investigated using spectroscopic and thermodynamic methods. Albumin fluorescence intensity was quenched by nanoparticles with maximum emission intensity decreasing at the initial reaction and increasing at the last reacted period. Binding constants (Kb) were 1.12 x 105 M-1, 4.12 x 105 M-1 and 7.44 x 105 M-1 to CHP-3.11, CHP-6.03 and CHP-6.91, respectively, as determined by Stern-Volmer analysis. Adsorption of albumin to nanoparticles was an exothermic reaction process and revealed a higher DS of cholesterol moiety with higher enthalpy and entropy changes. Upon interaction with nanoparticles, albumin conformation changed with a reduction of α-helix, suggesting a partial protein unfolding. Furthermore, albumin could gradually change its helical structure due to the structural change of the complexed nanoparticle. Particle hydrophobicity and shell-core structure play a main role in the alteration of albumin conformation in the nanoparticle-protein interaction process.
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Cite this article as:
Tao Xiaojun, Zhang Qiufang, Yang Wenzhi and Zhang Qiqing, The Interaction between Human Serum Albumin and Cholesterol-modified Pullulan Nanoparticle, Current Nanoscience 2012; 8 (6) . https://dx.doi.org/10.2174/157341312803989105
DOI https://dx.doi.org/10.2174/157341312803989105 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
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