Photobiostimulation on Wound Healing Treatment by ClAlPc-nanoemulsion from a Multiple-Wavelength Portable Light Source on a 3D-Human Stem Cell Dermal Equivalent

F.L.      Primo; L.B.      de Paula; M.P.      de Siqueira-Moura; A.C.      Tedesco

doi:10.2174/092986712803530502

Abstract

This research evaluated the effect of multiple-wave lasertherapy on the healing process of surgical wounds based on in vitro models denominated stem-dermal equivalents. These human skin models were obtained from a co-culture of dermal cells and bone marrow mesenchymal stem cells. The experimental tests were carried out using a LED portable to multiple waves (operating at 660 nm and 810 nm) at different doses to induce photobiostimulation (10 to 70 mJ.cm-2). Moreover, a photosensitizer drug was employed as a new advanced designed nanomaterial, being a nanoemulsion with biopolymers to obtain an efficient drug delivery system to release lipophilic compounds. The studies were performed considering the light combination application monitoring the kinetic contraction of the dermal equivalent model and the quantification of important macromolecules (as metaloproteases derivatives), related directly with wound healing process. Results showed that an appropriate photomodulation using the combination of both wavelengths (in the red and infrared range) is possible, such that it can contribute to wound healing therapy and/or other pathological skin disease treatment.

Keywords: 3D-dermal equivalents, mesenchymal stem cells, multiple-wave light source, nanobiotechnology, phthalocyanine, tissue engineering, zymography, photodynamic process, low-level-laser, human skin