New Cathepsin D Inhibitor Library Utilizing Hydroxyethyl Isosteres with Cyclic Tertiary Amines

Title:New Cathepsin D Inhibitor Library Utilizing Hydroxyethyl Isosteres with Cyclic Tertiary Amines

Volume: 8 Issue: 6

Author(s): Rose M. McConnell, Kalyani Inapudi, Naveen Kadasala, Karthika Yarlagadda, Priya Velusamy, Matthew S. McConnell, Adam Green, Carol Trana, Kelley Sayyar and James S. McConnell

Affiliation:

Keywords: Aspartyl protease, Cathepsin D, Inhibitors, Hydroxyethylamine, Piperazine, Pipecolinate, Piperazinocolinate

Abstract: The design and synthesis of hydroxyethylamine isosteres as inhibitors of cathepsin D based on SAR data have been accomplished. A library of 96 of these hydroxyethylamine isosteres are described and many have proven to be very potent inhibitors of human cathepsin D activity as measured using a fluorometric assay technique, via peptide substrate Ac-Glu-Glu(Edans)-Lys-Pro-Ile-Cys-Phe-Phe-Arg-Leu-Gly-Lys(Methyl Red)-Glu-NH2. Compounds showing strongest inhibition of cathepsin D activity were those that contain a hydroxyethyl-N’-2- or N’-(4-chlorophenyl)piperazine moiety (IC50 values range from 0.55 to 8.5 nM), with N’-(2-pyrimidyl)piperizine (IC50 values range from 0.5 to 21.6 nM), with NN’- L-piperazinocolinamide (IC50 values range from 0.001 – 0.25 nM), or N-N’-L-piperazinocolin-N-methylamide (IC50 values range from 0.015 – 7.3 nM) .

Cite this article as:

M. McConnell Rose, Inapudi Kalyani, Kadasala Naveen, Yarlagadda Karthika, Velusamy Priya, S. McConnell Matthew, Green Adam, Trana Carol, Sayyar Kelley and S. McConnell James, New Cathepsin D Inhibitor Library Utilizing Hydroxyethyl Isosteres with Cyclic Tertiary Amines, Medicinal Chemistry 2012; 8 (6) . https://dx.doi.org/10.2174/1573406411208061146