Study of Neonatal Exposure to Estrogenic and Androgenic Endocrine Disruptors by Normal-Phase HPLC

Title:Study of Neonatal Exposure to Estrogenic and Androgenic Endocrine Disruptors by Normal-Phase HPLC

Volume: 2 Issue: 2

Author(s): Masahiro Kuwada, Rei Kawashima, Kazuo Nakamura, Hideyo Hasumi and Jun Maki

Affiliation:

Keywords: Endocrine disruptor, neonatal exposure, ovary, oogenesis, 17 β-estradiol, estrogen receptor.

Abstract: Neonatal exposure to estrogenic and androgenic endocrine disruptors induces developmental abnormalities in the female reproductive system. To investigate whether neonatal exposure affects oogenesis in juvenile and pubertal ovary, Sprague-Dawley rat pups were given estrogen or various endocrine disruptors by a single injection on the day of birth at concentrations ranging between 2 mM to 40 mM, and sacrificed on day 21 (juvenile) or 50 (puberty). The ovaries were weighed and examined histologically at each stage. Further, 17 β-estradiol synthesis of oogenesis was analyzed using normal-phase high-performance liquid chromatography. Neonatal exposure to 17 β-estradiol, diethylstilbestrol and estrogen receptor inhibitors significantly reduced ovary weights, although estrogen receptor inhibitors were completely restored by 17 β-estradiol synthesis during puberty.

Cite this article as:

Kuwada Masahiro, Kawashima Rei, Nakamura Kazuo, Hasumi Hideyo and Maki Jun, Study of Neonatal Exposure to Estrogenic and Androgenic Endocrine Disruptors by Normal-Phase HPLC, Drug Delivery Letters 2012; 2 (2) . https://dx.doi.org/10.2174/2210304x11202020126