Antibiotic Activity of Antimicrobial Peptide Against Pseudomonads Isolated from a Patient with Gallstones

Title: Antibiotic Activity of Antimicrobial Peptide Against Pseudomonads Isolated from a Patient with Gallstones

Volume: 13 Issue: 1

Author(s): Y. Park, J. Soo Hahm and K.- S. Hahm

Affiliation:

Keywords: Antibiotic activity, HP (2-20), ciprofloxacin, Pseudomonas aeruginosa

Abstract: We investigated the in vitro antibiotic activity of the 19-amino acid antimicrobial peptide HP (2-20), derived from the N-terminus of Helicobacter pylori Ribosomal Protein L1 (RPL1), against antibiotic susceptible and resistant pathogens from a patient with gallstones. HP (2-20) was active against antibiotic-susceptible and antibiotic-resistant clinical isolates of pathogens from a patient with gallstones, but this peptide showed no hemolytic activity against normal human erythrocytes. HP (2-20) acted synergistically with ciprofloxacin against pathogenic bacteria. Fluorescence activated flow cytometry revealed that the effect of HP (2-20) was dependent on energy and salt concentration. In addition, scanning electron microscopy showed that HP (2-20) caused significant morphological alterations to the cell surface of pathogens. Using 16S rDNA sequences, we found that isolates from bile were 100% homologous to Pseudomonas aeruginosa. These findings suggest that HP (2-20) may be useful clinically as an antibiotic against acquired pathogens from patients with gallstones and against pathogens resistant to other antibiotics.

Cite this article as:

Park Y., Hahm Soo J. and Hahm S. K.-, Antibiotic Activity of Antimicrobial Peptide Against Pseudomonads Isolated from a Patient with Gallstones, Protein & Peptide Letters 2006; 13 (1) . https://dx.doi.org/10.2174/092986606774502018

DOI https://dx.doi.org/10.2174/092986606774502018	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305