Abstract
High density lipoprotein (HDL) is recognized as the major negative risk factor of cardiovascular disease and number of anti-atherogenic functions has been ascribed to HDL. HDL is an assembly of a neutral lipid core and an outer shell consisting of polar lipids and proteins. It has been defined many different ways based on various distinct properties including density flotation, protein composition, molecular size, and electrophoretic migration. Overall the studies characterizing HDL clearly demonstrate that it is a complex heterogeneous mixture of particles. Furthermore several studies convincingly demonstrated that certain populations of HDL particles have a distinct functionality suggesting that HDL may serve as a platform for assembly of protein complexes with very specific biological functions. Indeed recent proteomics studies described over 100 proteins associated with HDL. Here we review approaches to isolation and proteomic analysis of HDL and discuss potential problems associated with isolation methods which may confound our understanding of the relation of the HDL composition and its biological function.
Keywords: Lipoproteins, HDL isolation, lipoprotein particle separation, cardiovascular disease, mass spectrometry, Cholesteryl ester transfer protein, coronary artery disease, niacin, kallistatin, coagulation factor
Current Vascular Pharmacology
Title:Proteomics Investigations of HDL: Challenges and Promise
Volume: 10 Issue: 4
Author(s): Tomas Vaisar
Affiliation:
Keywords: Lipoproteins, HDL isolation, lipoprotein particle separation, cardiovascular disease, mass spectrometry, Cholesteryl ester transfer protein, coronary artery disease, niacin, kallistatin, coagulation factor
Abstract: High density lipoprotein (HDL) is recognized as the major negative risk factor of cardiovascular disease and number of anti-atherogenic functions has been ascribed to HDL. HDL is an assembly of a neutral lipid core and an outer shell consisting of polar lipids and proteins. It has been defined many different ways based on various distinct properties including density flotation, protein composition, molecular size, and electrophoretic migration. Overall the studies characterizing HDL clearly demonstrate that it is a complex heterogeneous mixture of particles. Furthermore several studies convincingly demonstrated that certain populations of HDL particles have a distinct functionality suggesting that HDL may serve as a platform for assembly of protein complexes with very specific biological functions. Indeed recent proteomics studies described over 100 proteins associated with HDL. Here we review approaches to isolation and proteomic analysis of HDL and discuss potential problems associated with isolation methods which may confound our understanding of the relation of the HDL composition and its biological function.
Export Options
About this article
Cite this article as:
Vaisar Tomas, Proteomics Investigations of HDL: Challenges and Promise, Current Vascular Pharmacology 2012; 10 (4) . https://dx.doi.org/10.2174/157016112800812755
DOI https://dx.doi.org/10.2174/157016112800812755 |
Print ISSN 1570-1611 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6212 |
Call for Papers in Thematic Issues
TREATMENT OF CARDIOVASCULAR DISEASE IN CHRONIC AND END STAGE KIDNEY DISEASE
Cardiovascular disease still remains the leading cause of death in Chronic and End Stage Kidney Disease, accounting for more than half of all deaths in dialysis patients. During the past decade, research has been focused on novel therapeutic agents that might delay or even reverse cardiovascular disease and vascular calcification, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Therapeutic Potential of TACE Inhibitors in Stroke
Current Drug Targets - CNS & Neurological Disorders State of the Heart: CMR in Coronary Artery Disease
Current Medical Imaging The Pathobiology of Endothelin-1 in Vein Graft Disease: Are ETA Receptor Antagonists the Solution to Prevent Vein Graft Failure?
Current Vascular Pharmacology Transcriptional and Non-transcriptional Regulation of Glucose Metabolism and Insulin Sensitivity through Vitamin D
Current Nutrition & Food Science A Classical QSAR Study on Some Platelet Aggregation Inhibitors
Mini-Reviews in Medicinal Chemistry The Endocannabinoid System and Heart Disease: The Role of Cannabinoid Receptor Type 2
Cardiovascular & Hematological Disorders-Drug Targets Role of Whole Plant Extract of Nelumbo nucifera Gaertn in the Treatment of Thrombolysis
Cardiovascular & Hematological Agents in Medicinal Chemistry Update on the Imaging Techniques in the Diagnosis of Diabetic Retinopathy
Current Diabetes Reviews Refining Genetic Approaches for Identifying Behavioral Loci
Current Genomics Pharmacology of Adenosine A2A Receptors and Therapeutic Applications
Current Topics in Medicinal Chemistry Protein Degradation Pathways after Brain Ischemia
Current Drug Targets Critical Care for the Respiratory Specialist: Sepsis, Delirium and Long- Term Cognitive Dysfunction: Prevention with the Combination of Vitamin C, Hydrocortisone and Thiamine
Current Respiratory Medicine Reviews Evaluation of Microsomal Incubation Conditions on CYP3A4-Mediated Metabolism of Cyclosporine A by a Statistical Experimental Design
Current Drug Metabolism HIV Protease Inhibitors Impact on Apoptosis
Medicinal Chemistry Lecithin (Phosphatidylcholine): Healthy Dietary Supplement or Dangerous Toxin?
The Natural Products Journal Effects of Medroxyprogesterone Acetate on the Risk of Cardiovascular Disease in Postmenopausal Women Receiving Estrogen
Vascular Disease Prevention (Discontinued) Coronavirus (COVID-19) and Employee Health
Coronaviruses Mutations of Mitochondrial DNA in Atherosclerosis and Atherosclerosis-Related Diseases
Current Pharmaceutical Design Formulation and In Vivo Evaluation of Ticagrelor Self-nanoemulsifying Drug Delivery Systems
Pharmaceutical Nanotechnology The Implications of Human Stem Cell Differentiation to Endothelial Cell Via Fluid Shear Stress in Cardiovascular Regenerative Medicine: A Review
Current Pharmaceutical Design